Molecular and cellular basis of radiation fibrosis

Purpose: Recent data from the literature and the experimental work of the authors clearly indicate that TGF- beta 1 is a key modulator of cellular events, for example, induction of terminal differentiation, resulting in radiation-induced fibrosis. Therefore, the present study analysed which cellular...

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Bibliographic Details
Published in:International journal of radiation biology 1998-04, Vol.73 (4), p.401-408
Main Authors: BURGER, A, LÖFFLER, H, BAMBERG, M, RODEMANN, H. P
Format: Article
Language:English
Subjects:
Animals
Antibodies - pharmacology
Biological and medical sciences
Cell Differentiation - drug effects
Cell physiology
Colony-Forming Units Assay
Effects of physical and chemical agents
Fibroblasts - cytology
Fibroblasts - drug effects
Fundamental and applied biological sciences. Psychology
Humans
Lung - cytology
Lung - drug effects
Molecular and cellular biology
Phenotype
Radiation Pneumonitis - etiology
Radiation Pneumonitis - pathology
Rats
Stem Cells - drug effects
Transforming Growth Factor beta - immunology
Transforming Growth Factor beta - pharmacology
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Summary:Purpose: Recent data from the literature and the experimental work of the authors clearly indicate that TGF- beta 1 is a key modulator of cellular events, for example, induction of terminal differentiation, resulting in radiation-induced fibrosis. Therefore, the present study analysed which cellular processes induced by exogeneously added TGF- beta could be responsible for the induction, development and manifestation of the fibrotic phenotype in culture. Materials and methods: Rat lung fibroblast cultures (passage 1) were used. As a function of treatment with TGF- beta and/or anti-TGF beta -antibody, the clonogenic activity and differentiation pattern were analysed by colony-formation assays. Results: It could be demonstrated that treatment of rat lung progenitor fibroblasts with TGF- beta 1 resulted in a pronounced shift in the differentiation pattern, i.e. induction of post-mitotic fibrocytes. This TGF- beta 1-dependent terminal differentiation could be abolished by simultaneous treatment with a neutralizing antibody directed against TGF- beta 1. Conclusions: The data presented indicate that TGF- beta 1 is one major candidate mediating the accelerated terminal differentiation of progenitor fibroblasts to post-mitotic functional fibrocytes, which results in the fibrotic phenotype of this cell system.
ISSN:0955-3002
1362-3095
DOI:10.1080/095530098142239