Paclitaxel cytotoxicity against human lung cancer cell lines increases with prolonged exposure durations

Paclitaxel blocks cells in G2-M, and this may result in a schedule-dependent effect on paclitaxel cytotoxicity. To test this hypothesis, we evaluated paclitaxel cytotoxicity in 28 human lung cancer cell lines. Fourteen of the cell lines were derived from patients with non-small cell lung cancer (NSC...

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Bibliographic Details
Published in:Clinical cancer research 1997-03, Vol.3 (3), p.449-454
Main Authors: GEORGIADIS, M. S, RUSSELL, E. K, GAZDAR, A. F, JOHNSON, B. E
Format: Article
Language:English
Subjects:
Antineoplastic agents
Biological and medical sciences
Carcinoma, Non-Small-Cell Lung - pathology
Carcinoma, Small Cell - pathology
Cell Cycle - drug effects
Cell Survival - drug effects
Chemotherapy
G2 Phase
Humans
Lung Neoplasms - pathology
Medical sciences
Mitosis
Paclitaxel - toxicity
Pharmacology. Drug treatments
Time Factors
Tumor Cells, Cultured
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Summary:Paclitaxel blocks cells in G2-M, and this may result in a schedule-dependent effect on paclitaxel cytotoxicity. To test this hypothesis, we evaluated paclitaxel cytotoxicity in 28 human lung cancer cell lines. Fourteen of the cell lines were derived from patients with non-small cell lung cancer (NSCLC), and 14 were from patients with small cell lung cancer (SCLC). All cell lines were exposed to a range of paclitaxel concentrations for durations of 3, 24, and 120 h, and cytotoxicity was measured with a tetrazolium-based assay. The median IC50 values for all 28 cell lines at exposure durations of 3, 24, and 120 h were >32 microM, 23 microM, and 0.38 microM, respectively. The median IC50 values for the NSCLC cell lines were >32 microM, 9.4 microM, and 0.027 microM at exposure durations of 3, 24, and 120 h, respectively. For the 14 SCLC cell lines, the median IC50 values were >32 microM, 25 microM, and 5.0 microM, respectively. Five of the 14 SCLC cell lines had IC50 values at 120 h of paclitaxel exposure that were 1000-fold less than the remaining SCLC cell lines. The median IC50 values for these five sensitive SCLC cell lines at 3-, 24-, and 120-h exposures were >32 microM, 23 microM, and
ISSN:1078-0432
1557-3265