High‐energy open‐fracture model with initial experience of fluorescence‐guided bone perfusion assessment

High‐energy orthopedic injuries cause severe damage to soft tissues and are prone to infection and healing complications, making them a challenge to manage. Further research is facilitated by a clinically relevant animal model with commensurate fracture severity and soft‐tissue damage, allowing eval...

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Bibliographic Details
Published in:Journal of orthopaedic research 2023-05, Vol.41 (5), p.1040-1048
Main Authors: Demidov, Valentin V., Clark, Megan A., Streeter, Samuel S., Sottosanti, Joseph S., Gitajn, Ida Leah, Elliott, Jonathan Thomas
Format: Article
Language:English
Subjects:
Animals
computed tomography
dynamic contrast‐enhanced fluorescence imaging
fluorescence‐guided surgery
Fractures, Open
high‐energy trauma
optical imaging
Perfusion
Reproducibility of Results
Retrospective Studies
Tibia - diagnostic imaging
tibia fracture
Tibial Fractures - diagnostic imaging
Treatment Outcome
X-Ray Microtomography
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Summary:High‐energy orthopedic injuries cause severe damage to soft tissues and are prone to infection and healing complications, making them a challenge to manage. Further research is facilitated by a clinically relevant animal model with commensurate fracture severity and soft‐tissue damage, allowing evaluation of novel treatment options and techniques. Here we report a reproducible, robust, and clinically relevant animal model of high‐energy trauma with extensive soft‐tissue damage, based on compressed air‐driven membrane rupture as the blast wave source. As proof‐of‐principle showing the reproducibility of the injury, we evaluate changes in tissue and bone perfusion for a range of different tibia fracture severities, using dynamic contrast‐enhanced fluorescence imaging and microcomputed tomography. We demonstrate that fluorescence tracer temporal profiles for skin, femoral vein, fractured bone, and paw reflect the increasing impact of more powerful blasts causing a range of Gustilo grade I–III injuries. The maximum fluorescence intensity of distal tibial bone following 0.1 mg/kg intravenous indocyanine green injection decreased by 35% (p 
ISSN:0736-0266
1554-527X
DOI:10.1002/jor.25443