Quantification of phnAc and nahAc in contaminated New Zealand soils by competitive PCR

Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based s...

Full description

Saved in:
Bibliographic Details
Published in:Applied and environmental microbiology 2000-05, Vol.66 (5), p.1814-1817
Main Authors: LAURIE, A. D, LLOYD-JONES, G
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Applied ecology
BACTERIA
BIODEGRADATION
Biodegradation, Environmental
Biological and medical sciences
Burkholderia
Burkholderia - classification
Burkholderia - genetics
Burkholderia - isolation & purification
Ecotoxicology, biological effects of pollution
Environmental Microbiology and Biodegradation
ENVIRONMENTAL SCIENCES
Fundamental and applied biological sciences. Psychology
GENES
Genes, Bacterial
GENOTYPE
LAND POLLUTION
Microbiology
nahAc gene
NAPHTHALENE
Naphthalenes - metabolism
NEW ZEALAND
PHENANTHRENE
Phenanthrenes - metabolism
phnAc gene
Polycyclic aromatic hydrocarbons
Polycyclic Aromatic Hydrocarbons - metabolism
POLYMERASE CHAIN REACTION
Polymerase Chain Reaction - methods
Pseudomonas putida
REMEDIAL ACTION
Soil Microbiology
Soil Pollutants
SOILS
Terrestrial environment, soil, air
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. We used the recently described phn genes of Burkholderia sp. strain RP007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degradation and compared this genotype with the genotypically distinct but ubiquitous nah-like class in different soils. Competitive PCR quantification of phnAc and nahAc, which encode the iron sulfur protein large (alpha) subunits of PAH dioxygenases in nah-like and phn catabolic operons, revealed that the phn genotype can have a greater ecological significance than the nah-like genotype.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.66.5.1814-1817.2000