Bioinformatics Analysis to Uncover the Potential Drug Targets Responsible for Mycobacterium tuberculosis Peptidoglycan and Lysine Biosynthesis

Drug-resistant tuberculosis (TB), which results mainly from the selection of naturally resistant strains of Mycobacterium tuberculosis (MTB) due to mismanaged treatment, poses a severe challenge to the global control of TB. Therefore, screening novel and unique drug targets against this pathogen is...

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Bibliographic Details
Published in:Bioinformatics and biology insights 2023-01, Vol.17, p.11779322231171774-11779322231171774
Main Authors: Ayu Eka Pitaloka, Dian, Izzati, Afifah, Rafa Amirah, Siti, Abdan Syakuran, Luqman, Muhammad Irham, Lalu, Darumas Putri, Athika, Adikusuma, Wirawan
Format: Article
Language:English
Subjects:
Bioinformatics
Biosynthesis
Drug resistance
Encyclopedias
Genomes
Localization
Lysine
Metabolic pathways
Metabolism
Mycobacterium tuberculosis
Network analysis
Original
Peptidoglycans
Protein interaction
Proteins
Qualitative analysis
Therapeutic targets
Tuberculosis
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Summary:Drug-resistant tuberculosis (TB), which results mainly from the selection of naturally resistant strains of Mycobacterium tuberculosis (MTB) due to mismanaged treatment, poses a severe challenge to the global control of TB. Therefore, screening novel and unique drug targets against this pathogen is urgently needed. The metabolic pathways of Homo sapiens and MTB were compared using the Kyoto Encyclopedia of Genes and Genomes tool, and further, the proteins that are involved in the metabolic pathways of MTB were subtracted and proceeded to protein-protein interaction network analysis, subcellular localization, drug ability testing, and gene ontology. The study aims to identify enzymes for the unique pathways for further screening to determine the feasibility of the therapeutic targets. The qualitative characteristics of 28 proteins identified as drug target candidates were studied. The results showed that 12 were cytoplasmic, 2 were extracellular, 12 were transmembrane, and 3 were unknown. Furthermore, druggability analysis revealed 14 druggable proteins, of which 12 were novel and responsible for MTB peptidoglycan and lysine biosynthesis. The novel targets obtained in this study are used to develop antimicrobial treatments against pathogenic bacteria. Future studies should further shed light on the clinical implementation to identify antimicrobial therapies against MTB.
ISSN:1177-9322
1177-9322
DOI:10.1177/11779322231171774