UHRF1 is essential for proper cytoplasmic architecture and function of mouse oocytes and derived embryos

Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a protein essential for the maintenance of DNA methylation in somatic cells. However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear functio...

Full description

Saved in:
Bibliographic Details
Published in:Life science alliance 2023-08, Vol.6 (8), p.e202301904
Main Authors: Uemura, Shuhei, Maenohara, Shoji, Inoue, Kimiko, Ogonuki, Narumi, Matoba, Shogo, Ogura, Atsuo, Kurumizaka, Mayuko, Yamagata, Kazuo, Sharif, Jafar, Koseki, Haruhiko, Ueda, Koji, Unoki, Motoko, Sasaki, Hiroyuki
Format: Article
Language:English
Subjects:
Animals
CCAAT-Enhancer-Binding Proteins - genetics
Cytosol
Endoplasmic Reticulum
Mice
Mitochondria
Oocytes
Proteomics
Ubiquitin-Protein Ligases - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ubiquitin-like with PHD and RING finger domains 1 (UHRF1) is a protein essential for the maintenance of DNA methylation in somatic cells. However, UHRF1 is predominantly localized in the cytoplasm of mouse oocytes and preimplantation embryos, where it may play a role unrelated to the nuclear function. We herein report that oocyte-specific KO results in impaired chromosome segregation, abnormal cleavage division, and preimplantation lethality of derived embryos. Our nuclear transfer experiment showed that the phenotype is attributable to cytoplasmic rather than nuclear defects of the zygotes. A proteomic analysis of KO oocytes revealed the down-regulation of proteins associated with microtubules including tubulins, which occurred independently of transcriptomic changes. Intriguingly, cytoplasmic lattices were disorganized, and mitochondria, endoplasmic reticulum, and components of the subcortical maternal complex were mislocalized. Thus, maternal UHRF1 regulates the proper cytoplasmic architecture and function of oocytes and preimplantation embryos, likely through a mechanism unrelated to DNA methylation.
ISSN:2575-1077
2575-1077
DOI:10.26508/lsa.202301904