Aberrant Double-Strand Break Repair in rad51 Mutants of Saccharomyces cerevisiae

A number of studies of Saccharomyces cerevisiae have revealed RAD51-independent recombination events. These include spontaneous and double-strand break-induced recombination between repeated sequences, and capture of a chromosome arm by break-induced replication. Although recombination between inver...

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Bibliographic Details
Published in:Molecular and cellular biology 2000-12, Vol.20 (24), p.9162-9172
Main Authors: Kang, Leslie E., Symington, Lorraine S.
Format: Article
Language:English
Subjects:
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA Damage
DNA Dynamics and Chromosome Structure
DNA Nucleotidyltransferases - genetics
DNA Nucleotidyltransferases - metabolism
DNA Repair
DNA, Fungal - genetics
DNA, Fungal - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
double-strand break repair
Fungal Proteins - genetics
Fungal Proteins - metabolism
Gene Conversion
Lac Operon - genetics
Models, Genetic
Mutation
Plasmids - genetics
Plasmids - metabolism
Rad1 protein
Rad10 protein
RAD51 gene
Rad51 Recombinase
Recombination, Genetic - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
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Summary:A number of studies of Saccharomyces cerevisiae have revealed RAD51-independent recombination events. These include spontaneous and double-strand break-induced recombination between repeated sequences, and capture of a chromosome arm by break-induced replication. Although recombination between inverted repeats is considered to be a conservative intramolecular event, the lack of requirement for RAD51 suggests that repair can also occur by a nonconservative mechanism. We propose a model forRAD51-independent recombination by one-ended strand invasion coupled to DNA synthesis, followed by single-strand annealing. The Rad1/Rad10 endonuclease is required to trim intermediates formed during single-strand annealing and thus was expected to be required forRAD51-independent events by this model. Double-strand break repair between plasmid-borne inverted repeats was less efficient in rad1 rad51 double mutants than in rad1 andrad51 strains. In addition, repair events were delayed and frequently associated with plasmid loss. Furthermore, the repair products recovered from the rad1 rad51 strain were primarily in the crossover configuration, inconsistent with conservative models for mitotic double-strand break repair.
ISSN:0270-7306
1098-5549
1098-5549
DOI:10.1128/MCB.20.24.9162-9172.2000