Recycling of bacterial RNA polymerase by the Swi2/Snf2 ATPase RapA

Free-living bacteria have regulatory systems that can quickly reprogram gene transcription in response to changes in the cellular environment. The RapA ATPase, a prokaryotic homolog of the eukaryotic Swi2/Snf2 chromatin remodeling complex, may facilitate such reprogramming, but the mechanisms by whi...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2023-07, Vol.120 (28), p.e2303849120-e2303849120
Main Authors: Inlow, Koe, Tenenbaum, Debora, Friedman, Larry J, Kondev, Jane, Gelles, Jeff
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
ATP
Bacteria
Biological Sciences
Chromatin remodeling
Deoxyribonucleic acid
DNA
DNA - metabolism
DNA-directed RNA polymerase
DNA-Directed RNA Polymerases - metabolism
E coli
Elongation
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Fluorescence
Fluorescence microscopy
Genomes
Intermediates
Kinetics
Nucleotide sequence
Physical Sciences
Ribonucleic acid
RNA
RNA polymerase
RNA, Bacterial - metabolism
Transcription elongation
Transcription initiation
Transcription termination
Transcription, Genetic
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Summary:Free-living bacteria have regulatory systems that can quickly reprogram gene transcription in response to changes in the cellular environment. The RapA ATPase, a prokaryotic homolog of the eukaryotic Swi2/Snf2 chromatin remodeling complex, may facilitate such reprogramming, but the mechanisms by which it does so are unclear. We used multiwavelength single-molecule fluorescence microscopy in vitro to examine RapA function in the transcription cycle. In our experiments, RapA at
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2303849120