Enhancing Spns2/S1P in macrophages alleviates hyperinflammation and prevents immunosuppression in sepsis

• Published in: EMBO reports 2023-08, Vol.24 (8), p.e56635-n/a
• Main Authors: Fang, Chao, Ren, Pan, Bian, Ganlan, Wang, Jian, Bai, Jiaxin, Huang, Jiaxing, Ding, Yixiao, Li, Xueyong, Li, Mingkai, Hou, Zheng
• Format: Article
• Language: English
• Subjects: Anion Transport Proteins - metabolism; Antiinfectives and antibacterials; Defense mechanisms; EMBO19; EMBO21; EMBO37; Glycolysis; Homeostasis; Humans; Immune response; Immune system; Immunomodulation; Immunosuppression; Immunosuppression Therapy; Inflammation; Inflammatory response; Intracellular; Lactates; Lactic acid; Life Sciences; Macrophages; Macrophages - metabolism; Metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Sepsis; sphingosine‐1‐phosphate; Spinster homolog 2; Therapeutic targets
• ISSN: 1469-221X; 1469-3178; 1469-3178
• DOI: 10.15252/embr.202256635

Sepsis is a leading cause of in‐hospital mortality resulting from a dysregulated response to infection. Novel immunomodulatory therapies targeting macrophage metabolism have emerged as an important focus for current sepsis research. However, understanding the mechanisms underlying macrophage metabolic reprogramming and how they impact immune response requires further investigation. Here, we identify macrophage‐expressed Spinster homolog 2 (Spns2), a major transporter of sphingosine‐1‐phosphate (S1P), as a crucial metabolic mediator that regulates inflammation through the lactate‐reactive oxygen species (ROS) axis. Spns2 deficiency in macrophages significantly enhances glycolysis, thereby increasing intracellular lactate production. As a key effector, intracellular lactate promotes pro‐inflammatory response by increasing ROS generation. The overactivity of the lactate‐ROS axis drives lethal hyperinflammation during the early phase of sepsis. Furthermore, diminished Spns2/S1P signaling impairs the ability of macrophages to sustain an antibacterial response, leading to significant innate immunosuppression in the late stage of infection. Notably, reinforcing Spns2/S1P signaling contributes to balancing the immune response during sepsis, preventing both early hyperinflammation and later immunosuppression, making it a promising therapeutic target for sepsis. Synopsis Spns2/S1P signaling regulates macrophage metabolism and modulates the early antibacterial response through the lactate‐ROS axis. Macrophages also rely on Spns2/S1P signaling to prevent immunosuppression in the late phase of sepsis. Spns2/S1P signaling regulates mitochondrial dynamics by enhancing the malate–aspartate shuttle. Macrophage deficient for Spns2 shows overactivity of the lactate‐ROS axis and hyperinflammation in early sepsis. Diminished Spns2/S1P signaling potentially contributes to immunosuppression during the late phase of sepsis. Graphical Abstract Spns2/S1P signaling regulates macrophage metabolism and modulates the early antibacterial response through the lactate‐ROS axis. Macrophages also rely on Spns2/S1P signaling to prevent immunosuppression in the late phase of sepsis.