Activation of CWI pathway through high hydrostatic pressure, enhancing glycerol efflux via the aquaglyceroporin Fps1 in Saccharomyces cerevisiae

The fungal cell wall is the initial barrier for the fungi against diverse external stresses, such as osmolarity changes, harmful drugs, and mechanical injuries. This study explores the roles of osmoregulation and the cell-wall integrity (CWI) pathway in response to high hydrostatic pressure in the y...

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Published in:Molecular biology of the cell 2023-08, Vol.34 (9), p.ar92-ar92
Main Authors: Mochizuki, Takahiro, Tanigawa, Toshiki, Shindo, Seiya, Suematsu, Momoka, Oguchi, Yuki, Mioka, Tetsuo, Kato, Yusuke, Fujiyama, Mina, Hatano, Eri, Yamaguchi, Masashi, Chibana, Hiroji, Abe, Fumiyoshi
Format: Article
Language:English
Subjects:
Analysis
Aquaglyceroporins - metabolism
Aquaporins
Brewer's yeast
Cell Wall - metabolism
Glycerol - metabolism
Hydrostatic Pressure
Identification and classification
Methods
Phosphorylation
Plant cell walls
Properties
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - metabolism
Special Issue on Forces On And Within Cells
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Summary:The fungal cell wall is the initial barrier for the fungi against diverse external stresses, such as osmolarity changes, harmful drugs, and mechanical injuries. This study explores the roles of osmoregulation and the cell-wall integrity (CWI) pathway in response to high hydrostatic pressure in the yeast . We demonstrate the roles of the transmembrane mechanosensor Wsc1 and aquaglyceroporin Fps1 in a general mechanism to maintain cell growth under high-pressure regimes. The promotion of water influx into cells at 25 MPa, as evident by an increase in cell volume and a loss of the plasma membrane eisosome structure, activates the CWI pathway through the function of Wsc1. Phosphorylation of Slt2, the downstream mitogen-activated protein kinase, was increased at 25 MPa. Glycerol efflux increases via Fps1 phosphorylation, which is initiated by downstream components of the CWI pathway, and contributes to the reduction in intracellular osmolarity under high pressure. The elucidation of the mechanisms underlying adaptation to high pressure through the well-established CWI pathway could potentially translate to mammalian cells and provide novel insights into cellular mechanosensation.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E23-03-0086