Endoplasmic reticulum stress controls PIN-LIKES abundance and thereby growth adaptation

• Published in: Proceedings of the National Academy of Sciences - PNAS 2023-08, Vol.120 (31), p.e2218865120-e2218865120
• Main Authors: Waidmann, Sascha, Béziat, Chloé, Ferreira Da Silva Santos, Jonathan, Feraru, Elena, Feraru, Mugurel I, Sun, Lin, Noura, Seinab, Boutté, Yohann, Kleine-Vehn, Jürgen
• Format: Article
• Language: English
• Subjects: Adaptation; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Auxins; Biochemistry, Molecular Biology; Biological Sciences; Biological Transport; Botanics; Endoplasmic reticulum; Endoplasmic Reticulum Stress; Environmental conditions; Gene Expression Regulation, Plant; Genetics; Genomics; Hypocotyls; Indoleacetic Acids - metabolism; Life Sciences; Lipids; Membrane proteins; Membrane Transport Proteins - metabolism; Plant growth; Plant Growth Regulators - metabolism; Plant hormones; Plants - metabolism; Protein turnover; Proteins; Vegetal Biology
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2218865120

Extreme environmental conditions eventually limit plant growth [J. R. Dinneny, , 1-19 (2019), N. Gigli-Bisceglia, C. Testerink, , 102120 (2021)]. Here, we reveal a mechanism that enables multiple external cues to get integrated into auxin-dependent growth programs in . Our forward genetics approach on dark-grown hypocotyls uncovered that an imbalance in membrane lipids enhances the protein abundance of PIN-LIKES (PILS) [E. Barbez , , 119 (2012)] auxin transport facilitators at the endoplasmic reticulum (ER), which thereby limits nuclear auxin signaling and growth rates. We show that this subcellular response relates to ER stress signaling, which directly impacts PILS protein turnover in a tissue-dependent manner. This mechanism allows PILS proteins to integrate environmental input with phytohormone auxin signaling, contributing to stress-induced growth adaptation in plants.