Specific mRNA lipid nanoparticles and acquired resistance to ticks

Acquired resistance to ticks can develop when animals are repeatedly exposed to ticks. Recently, acquired resistance to Ixodes scapularis was induced in guinea pigs immunized with an mRNA-lipid nanoparticle vaccine (19ISP) encoding 19 I. scapularis proteins. Here, we evaluated specific mRNAs present...

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Bibliographic Details
Published in:Vaccine 2023-07, Vol.41 (34), p.4996-5002
Main Authors: Matias, Jaqueline, Cui, Yingjun, Tang, Xiaotian, Sajid, Andaleeb, Arora, Gunjan, Wu, Ming-Jie, DePonte, Kathleen, Muramatsu, Hiromi, Tam, Ying K., Narasimhan, Sukanya, Pardi, Norbert, Weissman, Drew, Fikrig, Erol
Format: Article
Language:English
Subjects:
Acquired resistance to ticks
Animals
Antibodies
Antigens
Arachnids
Critical components
Erythema
Guinea Pigs
Immunization
Insect bites
Ixodes - genetics
Ixodes scapularis
Laboratory animals
Lipids
Lyme Disease
mRNA
mRNA vaccine
Nanoparticles
Pathogens
Proteins
RNA, Messenger
Saliva
Statistical analysis
Ticks
Vaccines
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Summary:Acquired resistance to ticks can develop when animals are repeatedly exposed to ticks. Recently, acquired resistance to Ixodes scapularis was induced in guinea pigs immunized with an mRNA-lipid nanoparticle vaccine (19ISP) encoding 19 I. scapularis proteins. Here, we evaluated specific mRNAs present in 19ISP to identify critical components associated with resistance to ticks. A lipid nanoparticle containing 12 mRNAs which included all the targets within 19ISP that elicited strong humoral responses in guinea pigs, was sufficient to induce robust resistance to ticks. Lipid nanoparticles containing fewer mRNAs or a single mRNA were not able to generate strong resistance to ticks. All lipid nanoparticles containing salp14 mRNA, however, were associated with increased redness at the tick bite site – which is the first manifestation of acquired resistance to ticks. This study demonstrates that more than one I. scapularis target within 19ISP is required for resistance to ticks, and that additional targets may also play a role in this process.
ISSN:0264-410X
1873-2518
1873-2518
DOI:10.1016/j.vaccine.2023.06.081