Mycobacterial RNase E cleaves with a distinct sequence preference and controls the degradation rates of most Mycolicibacterium smegmatis mRNAs

• Published in: The Journal of biological chemistry 2023-11, Vol.299 (11), p.105312-105312, Article 105312
• Main Authors: Zhou, Ying, Sun, Huaming, Rapiejko, Abigail R., Vargas-Blanco, Diego A., Martini, Maria Carla, Chase, Michael R., Joubran, Samantha R., Davis, Alexa B., Dainis, Joseph P., Kelly, Jessica M., Ioerger, Thomas R., Roberts, Louis A., Fortune, Sarah M., Shell, Scarlet S.
• Format: Article
• Language: English
• Subjects: Collection: RNA Sequencing; Mycobacterium smegmatis; Mycobacterium smegmatis - enzymology; Mycobacterium smegmatis - metabolism; Mycobacterium tuberculosis; Mycobacterium tuberculosis - metabolism; Mycolicibacterium smegmatis; RNA degradation; RNA processing; RNA, Bacterial - metabolism; RNA, Messenger - metabolism; RNase E
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/j.jbc.2023.105312

The mechanisms and regulation of RNA degradation in mycobacteria have been subject to increased interest following the identification of interplay between RNA metabolism and drug resistance. Mycobacteria encode multiple ribonucleases predicted to participate in mRNA degradation and/or processing of stable RNAs. RNase E is an endoribonuclease hypothesized to play a major role in mRNA degradation because of its essentiality in mycobacteria and its role in mRNA degradation in gram-negative bacteria. Here, we defined the impact of RNase E on mRNA degradation rates transcriptome wide in the nonpathogenic model Mycolicibacterium smegmatis. RNase E played a rate-limiting role in degradation of the transcripts encoded by at least 89% of protein-coding genes, with leadered transcripts often being more affected by RNase E repression than leaderless transcripts. There was an apparent global slowing of transcription in response to knockdown of RNase E, suggesting that M. smegmatis regulates transcription in responses to changes in mRNA degradation. This compensation was incomplete, as the abundance of most transcripts increased upon RNase E knockdown. We assessed the sequence preferences for cleavage by RNase E transcriptome wide in M. smegmatis and Mycobacterium tuberculosis and found a consistent bias for cleavage in C-rich regions. Purified RNase E had a clear preference for cleavage immediately upstream of cytidines, distinct from the sequence preferences of RNase E in gram-negative bacteria. We furthermore report a high-resolution map of mRNA cleavage sites in M. tuberculosis, which occur primarily within the RNase E-preferred sequence context, confirming that RNase E has a broad impact on the M. tuberculosis transcriptome.