Plant dihydroxyacetone phosphate reductases: Purification, characterization, and localization

A cytosolic form of dihydroxyacetone phosphate (DHAP) reductase was purified 200,000-fold from spinach (Spinacia oleracea L.) leaves to apparent electrophoretic homogeneity. The purification procedure included anion-exchange chromatography, gel filtration, hydrophobic chromatography, and dye-ligand...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1992-09, Vol.100 (1), p.352-359
Main Authors: Kirsch, T, Gerber, D.W, Byerrum, R.U, Tolbert, N.E
Format: Article
Language:English
Subjects:
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
Agronomy. Soil science and plant productions
ALCOHOL DESHIDROGENASA
ALCOOL DESHYDROGENASE
BIOCHIMIE
Biological and medical sciences
BIOQUIMICA
CHLOROPLASTE
Chloroplasts
Chromatography
CITOPLASMA
CLOROPLASTO
CYTOPLASME
Dihydroxyacetone phosphate
Enzymes
Fundamental and applied biological sciences. Psychology
Gels
Metabolism
Metabolism and Enzymology
Peroxisomes
Phosphates
Plant physiology and development
Plants
Protein isoforms
PURIFICACION
PURIFICATION
Spinach
SPINACIA OLERACEA
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Summary:A cytosolic form of dihydroxyacetone phosphate (DHAP) reductase was purified 200,000-fold from spinach (Spinacia oleracea L.) leaves to apparent electrophoretic homogeneity. The purification procedure included anion-exchange chromatography, gel filtration, hydrophobic chromatography, and dye-ligand chromatography on Green-A and Red-A agaroses. The enzyme, prepared in an overall yield of 14%, had a final specific activity of about 500, micromole of DHAP reduced min-1 mg-1 protein, a subunit molecular mass of 38 kD, and a native molecular mass of 75 kD. A chloroplastic isoform of DHAP reductase was separated from the cytosolic form by anion-exchange chromatography and partially purified 56,000 -old to a specific activity of 135 micromole min-1 mg-1 protein. Antibodies generated in rabbits against the cytosolic form did not crossreact with the chloroplastic isoform. The two reductases were specific for NADH and DHAP. Although they exhibited some dissimilarities, both isoforms were severely inhibited by higher molecular weight fatty acyl coenzyme A esters and phosphohydroxypyruvate and moderately inhibited by nucleotides. In contrast to previous reports, the partially purified chloroplastic enzyme was not stimulated by dithiothreitol or thioredoxin, nor was the purified cytosolic enzyme stimulated by fructose 2,6-bisphosphate. A third DHAP reductase isoform was isolated from spinach leaf peroxisomes that had been prepared by isopycnic sucrose density gradient centrifugation. The peroxisomal DHAP reductase was sensitive to antibodies raised against the cytosolic enzyme and had a slightly smaller subunit molecular weight than the cytosolic isoform
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.100.1.352