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Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds

A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequ...

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Published in:	Plant physiology (Bethesda) 1990-11, Vol.94 (3), p.1488-1491
Main Authors:	Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan), Tanaka, Y, Morohashi, Y
Format:	Article
Language:	English
Subjects:	ALFA AMILASA ALPHA AMYLASE Amino acids Barley Biological and medical sciences BIOSINTESIS BIOSYNTHESE CLONE CLONES CODE GENETIQUE CODIGO GENETICO Communications Complementary DNA COTILEDONES COTYLEDON Cotyledons DNA Enzymes EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENETICA GENETIQUE Germination Imbibition Messenger RNA Metabolism Molecular Biology and Gene Regulation Plant physiology and development RNA Testa VIGNA RADIATA
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container_title	Plant physiology (Bethesda)
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creator	Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan) Tanaka, Y Morohashi, Y
description	A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequence of the purified alpha-amylase, it was inferred that 23 N-terminal amino acids of a nascent polypeptide represent a signal peptide. Northern blot analysis showed that the levels of alpha-amylase mRNA are in parallel with the activities of alpha-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during inhibition, a rapid increase in the amount of the alpha-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the alpha-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage
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Northern blot analysis showed that the levels of alpha-amylase mRNA are in parallel with the activities of alpha-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during inhibition, a rapid increase in the amount of the alpha-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the alpha-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.94.3.1488</identifier><identifier>PMID: 16667859</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ALFA AMILASA ; ALPHA AMYLASE ; Amino acids ; Barley ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; CLONE ; CLONES ; CODE GENETIQUE ; CODIGO GENETICO ; Communications ; Complementary DNA ; COTILEDONES ; COTYLEDON ; Cotyledons ; DNA ; Enzymes ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENETICA ; GENETIQUE ; Germination ; Imbibition ; Messenger RNA ; Metabolism ; Molecular Biology and Gene Regulation ; Plant physiology and development ; RNA ; Testa ; VIGNA RADIATA</subject><ispartof>Plant physiology (Bethesda), 1990-11, Vol.94 (3), p.1488-1491</ispartof><rights>Copyright 1990 American Society of Plant Physiologists</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4273263$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4273263$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,58213,58446</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19763739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16667859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan)</creatorcontrib><creatorcontrib>Tanaka, Y</creatorcontrib><creatorcontrib>Morohashi, Y</creatorcontrib><title>Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequence of the purified alpha-amylase, it was inferred that 23 N-terminal amino acids of a nascent polypeptide represent a signal peptide. Northern blot analysis showed that the levels of alpha-amylase mRNA are in parallel with the activities of alpha-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during inhibition, a rapid increase in the amount of the alpha-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the alpha-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage</description><subject>ALFA AMILASA</subject><subject>ALPHA AMYLASE</subject><subject>Amino acids</subject><subject>Barley</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>CLONE</subject><subject>CLONES</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>Communications</subject><subject>Complementary DNA</subject><subject>COTILEDONES</subject><subject>COTYLEDON</subject><subject>Cotyledons</subject><subject>DNA</subject><subject>Enzymes</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Germination</subject><subject>Imbibition</subject><subject>Messenger RNA</subject><subject>Metabolism</subject><subject>Molecular Biology and Gene Regulation</subject><subject>Plant physiology and development</subject><subject>RNA</subject><subject>Testa</subject><subject>VIGNA RADIATA</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkU1v1DAQhiMEokvhxgkh5AviQhZ_xXEuSKvyVakCCejZmjjO1pVjBzsp2v_Ej8RhV7vUF9t6n3lmpCmK5wSvCcH83TiuG75ma8KlfFCsSMVoSSsuHxYrjPMbS9mcFU9SusUYE0b44-KMCCFqWTWr4s9lCg4mGzwKPQKkP3zdIO2CN6gPEYEbb6CEYecgGWQ9Gma_Ra0Bj3SYds50wac12nhwu2TTP8e9kuF79jlzZ1xayk9FqJujzS7wXe7kXPi9_LYmDtYf5zl1S8Z06WnxqAeXzLPDfV5cf_r48-JLefXt8-XF5qrUVFZTyZhgvaixpHXXtZ0xWEDfcEYlwVLTtiZAtawk9Plo0rOKt60QbVVz3Ght2Hnxfu8d53YwnTZ-iuDUGO0AcacCWHU_8fZGbcOdIrjODp4Fbw6CGH7NJk1qsEkb58CbMCdVM8YbXuEmk2_3pI4hpWj6YxeC1bJfNY6q4YqpZb8Zf_X_ZCf4sNAMvD4AkDS4PoLXNp24phasZgv3cs_dpinEY85pzahgOX6xj3sICrYxK65_NIRQUVH2F5yvw5o</recordid><startdate>19901101</startdate><enddate>19901101</enddate><creator>Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan)</creator><creator>Tanaka, Y</creator><creator>Morohashi, Y</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19901101</creationdate><title>Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds</title><author>Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan) ; Tanaka, Y ; Morohashi, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c285t-3363f670827ddbdee06af94328108c2b71a2c858affffc1f354bb66b57409cce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>ALFA AMILASA</topic><topic>ALPHA AMYLASE</topic><topic>Amino acids</topic><topic>Barley</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>CLONE</topic><topic>CLONES</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>Communications</topic><topic>Complementary DNA</topic><topic>COTILEDONES</topic><topic>COTYLEDON</topic><topic>Cotyledons</topic><topic>DNA</topic><topic>Enzymes</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Germination</topic><topic>Imbibition</topic><topic>Messenger RNA</topic><topic>Metabolism</topic><topic>Molecular Biology and Gene Regulation</topic><topic>Plant physiology and development</topic><topic>RNA</topic><topic>Testa</topic><topic>VIGNA RADIATA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan)</creatorcontrib><creatorcontrib>Tanaka, Y</creatorcontrib><creatorcontrib>Morohashi, Y</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koizuka, N. (Tokyo University of Agriculture and Technology, Tokyo, Japan)</au><au>Tanaka, Y</au><au>Morohashi, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1990-11-01</date><risdate>1990</risdate><volume>94</volume><issue>3</issue><spage>1488</spage><epage>1491</epage><pages>1488-1491</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A cDNA was isolated that codes for alpha-amylase in mung bean (Vigna radiata) cotyledons, and the nucleotide sequence was determined. The deduced amino acid sequence (421 amino acid residues) is about 65% homologous with those of barley alpha-amylases. By comparing the deduced sequence with the sequence of the purified alpha-amylase, it was inferred that 23 N-terminal amino acids of a nascent polypeptide represent a signal peptide. Northern blot analysis showed that the levels of alpha-amylase mRNA are in parallel with the activities of alpha-amylase synthesis in cotyledons. Under the conditions where the solute leakage from cotyledons is accelerated during inhibition, a rapid increase in the amount of the alpha-amylase mRNA occurs. We postulate that a factor(s) which regulates in an inhibitory manner the alpha-amylase expression at the transcriptional level may be present in dry cotyledons and be removed by leakage</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>16667859</pmid><doi>10.1104/pp.94.3.1488</doi><tpages>4</tpages></addata></record>
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source	JSTOR Archival Journals and Primary Sources Collection; Alma/SFX Local Collection
subjects	ALFA AMILASA ALPHA AMYLASE Amino acids Barley Biological and medical sciences BIOSINTESIS BIOSYNTHESE CLONE CLONES CODE GENETIQUE CODIGO GENETICO Communications Complementary DNA COTILEDONES COTYLEDON Cotyledons DNA Enzymes EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENETICA GENETIQUE Germination Imbibition Messenger RNA Metabolism Molecular Biology and Gene Regulation Plant physiology and development RNA Testa VIGNA RADIATA
title	Isolation of a cDNA clone for alpha-amylase in mung bean cotyledons. Analysis of alpha-amylase mRNA levels in cotyledons during and following germination of mung bean seeds
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