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Genome editing in rice using CRISPR/Cas12i3
Summary The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIP...
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| Published in: | Plant biotechnology journal 2024-02, Vol.22 (2), p.379-385 |
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| cited_by | cdi_FETCH-LOGICAL-c4982-48ff6ac6c7b4993f11889062ff73b552aedcf5ca79199c07d8cd20ca376ac2c63 |
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| cites | cdi_FETCH-LOGICAL-c4982-48ff6ac6c7b4993f11889062ff73b552aedcf5ca79199c07d8cd20ca376ac2c63 |
| container_end_page | 385 |
| container_issue | 2 |
| container_start_page | 379 |
| container_title | Plant biotechnology journal |
| container_volume | 22 |
| creator | Lv, Ping Su, Fei Chen, Fangyuan Yan, Chunxue Xia, Dandan Sun, Hui Li, Shanshan Duan, Zhiqiang Ma, Changle Zhang, Hui Wang, Mugui Niu, Xiaomu Zhu, Jian‐Kang Zhang, Jinshan |
| description | Summary
The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants. |
| doi_str_mv | 10.1111/pbi.14192 |
| format | article |
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The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants.</description><identifier>ISSN: 1467-7644</identifier><identifier>ISSN: 1467-7652</identifier><identifier>EISSN: 1467-7652</identifier><identifier>DOI: 10.1111/pbi.14192</identifier><identifier>PMID: 37822083</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Arrays ; Base editors ; CRISPR ; CRISPR-Cas Systems - genetics ; CRISPR/Cas12i3 ; DNA structural variations ; Editing ; Efficiency ; Endonuclease ; Endonucleases - genetics ; Gene Editing - methods ; Gene expression ; Genome editing ; Genomes ; Herbicides ; Multiplex DR‐spacer Array ; Mutation ; Nuclease ; Oryza - genetics ; Plant breeding ; Plants - genetics ; Rice</subject><ispartof>Plant biotechnology journal, 2024-02, Vol.22 (2), p.379-385</ispartof><rights>2023 The Authors. published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2023 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.</rights><rights>2024. This work is published under http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4982-48ff6ac6c7b4993f11889062ff73b552aedcf5ca79199c07d8cd20ca376ac2c63</citedby><cites>FETCH-LOGICAL-c4982-48ff6ac6c7b4993f11889062ff73b552aedcf5ca79199c07d8cd20ca376ac2c63</cites><orcidid>0000-0003-3360-2799 ; 0000-0002-4929-8317 ; 0000-0001-7360-6837</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2919802372/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2919802372?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,11561,25752,27923,27924,37011,37012,44589,46051,46475,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37822083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lv, Ping</creatorcontrib><creatorcontrib>Su, Fei</creatorcontrib><creatorcontrib>Chen, Fangyuan</creatorcontrib><creatorcontrib>Yan, Chunxue</creatorcontrib><creatorcontrib>Xia, Dandan</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Duan, Zhiqiang</creatorcontrib><creatorcontrib>Ma, Changle</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Wang, Mugui</creatorcontrib><creatorcontrib>Niu, Xiaomu</creatorcontrib><creatorcontrib>Zhu, Jian‐Kang</creatorcontrib><creatorcontrib>Zhang, Jinshan</creatorcontrib><title>Genome editing in rice using CRISPR/Cas12i3</title><title>Plant biotechnology journal</title><addtitle>Plant Biotechnol J</addtitle><description>Summary
The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants.</description><subject>Arrays</subject><subject>Base editors</subject><subject>CRISPR</subject><subject>CRISPR-Cas Systems - genetics</subject><subject>CRISPR/Cas12i3</subject><subject>DNA structural variations</subject><subject>Editing</subject><subject>Efficiency</subject><subject>Endonuclease</subject><subject>Endonucleases - genetics</subject><subject>Gene Editing - methods</subject><subject>Gene expression</subject><subject>Genome editing</subject><subject>Genomes</subject><subject>Herbicides</subject><subject>Multiplex DR‐spacer Array</subject><subject>Mutation</subject><subject>Nuclease</subject><subject>Oryza - genetics</subject><subject>Plant breeding</subject><subject>Plants - genetics</subject><subject>Rice</subject><issn>1467-7644</issn><issn>1467-7652</issn><issn>1467-7652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>PIMPY</sourceid><recordid>eNp1kU1LwzAAhoMobk4P_gEpeFGkWz7afJxEi87BwDH1HNI0nRldO5tV2b83s3OoYC5JyJOHN3kBOEWwj_wYLFPbRxESeA90UURZyGiM93frKOqAI-fmEGJEY3oIOoRxjCEnXXA1NGW1MIHJ7MqWs8CWQW21CRq32SXT0dNkOkiUQ9iSY3CQq8KZk-3cAy_3d8_JQzh-HI6Sm3GoI8FxGPE8p0pTzdJICJIjxLmAFOc5I2kcY2UyncdaMYGE0JBlXGcYakWYv4U1JT1w3XqXTbrwsClXtSrksrYLVa9lpaz8fVLaVzmr3iWCHFMhNoaLraGu3hrjVnJhnTZFoUpTNU5iziglnFPk0fM_6Lxq6tK_T2IfkENMGPbUZUvpunKuNvkuDYJy04H0HcivDjx79jP-jvz-dA8MWuDDFmb9v0lObket8hMyoY4S</recordid><startdate>202402</startdate><enddate>202402</enddate><creator>Lv, Ping</creator><creator>Su, Fei</creator><creator>Chen, Fangyuan</creator><creator>Yan, Chunxue</creator><creator>Xia, Dandan</creator><creator>Sun, Hui</creator><creator>Li, Shanshan</creator><creator>Duan, Zhiqiang</creator><creator>Ma, Changle</creator><creator>Zhang, Hui</creator><creator>Wang, Mugui</creator><creator>Niu, Xiaomu</creator><creator>Zhu, Jian‐Kang</creator><creator>Zhang, Jinshan</creator><general>John Wiley & Sons, Inc</general><general>John Wiley and Sons Inc</general><scope>24P</scope><scope>WIN</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-3360-2799</orcidid><orcidid>https://orcid.org/0000-0002-4929-8317</orcidid><orcidid>https://orcid.org/0000-0001-7360-6837</orcidid></search><sort><creationdate>202402</creationdate><title>Genome editing in rice using CRISPR/Cas12i3</title><author>Lv, Ping ; Su, Fei ; Chen, Fangyuan ; Yan, Chunxue ; Xia, Dandan ; Sun, Hui ; Li, Shanshan ; Duan, Zhiqiang ; Ma, Changle ; Zhang, Hui ; Wang, Mugui ; Niu, Xiaomu ; Zhu, Jian‐Kang ; Zhang, Jinshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4982-48ff6ac6c7b4993f11889062ff73b552aedcf5ca79199c07d8cd20ca376ac2c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Arrays</topic><topic>Base editors</topic><topic>CRISPR</topic><topic>CRISPR-Cas Systems - genetics</topic><topic>CRISPR/Cas12i3</topic><topic>DNA structural variations</topic><topic>Editing</topic><topic>Efficiency</topic><topic>Endonuclease</topic><topic>Endonucleases - genetics</topic><topic>Gene Editing - methods</topic><topic>Gene expression</topic><topic>Genome editing</topic><topic>Genomes</topic><topic>Herbicides</topic><topic>Multiplex DR‐spacer Array</topic><topic>Mutation</topic><topic>Nuclease</topic><topic>Oryza - genetics</topic><topic>Plant breeding</topic><topic>Plants - genetics</topic><topic>Rice</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lv, Ping</creatorcontrib><creatorcontrib>Su, Fei</creatorcontrib><creatorcontrib>Chen, Fangyuan</creatorcontrib><creatorcontrib>Yan, Chunxue</creatorcontrib><creatorcontrib>Xia, Dandan</creatorcontrib><creatorcontrib>Sun, Hui</creatorcontrib><creatorcontrib>Li, Shanshan</creatorcontrib><creatorcontrib>Duan, Zhiqiang</creatorcontrib><creatorcontrib>Ma, Changle</creatorcontrib><creatorcontrib>Zhang, Hui</creatorcontrib><creatorcontrib>Wang, Mugui</creatorcontrib><creatorcontrib>Niu, Xiaomu</creatorcontrib><creatorcontrib>Zhu, Jian‐Kang</creatorcontrib><creatorcontrib>Zhang, Jinshan</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Wiley Online Library Free Content</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant biotechnology journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lv, Ping</au><au>Su, Fei</au><au>Chen, Fangyuan</au><au>Yan, Chunxue</au><au>Xia, Dandan</au><au>Sun, Hui</au><au>Li, Shanshan</au><au>Duan, Zhiqiang</au><au>Ma, Changle</au><au>Zhang, Hui</au><au>Wang, Mugui</au><au>Niu, Xiaomu</au><au>Zhu, Jian‐Kang</au><au>Zhang, Jinshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome editing in rice using CRISPR/Cas12i3</atitle><jtitle>Plant biotechnology journal</jtitle><addtitle>Plant Biotechnol J</addtitle><date>2024-02</date><risdate>2024</risdate><volume>22</volume><issue>2</issue><spage>379</spage><epage>385</epage><pages>379-385</pages><issn>1467-7644</issn><issn>1467-7652</issn><eissn>1467-7652</eissn><abstract>Summary
The CRISPR/Cas type V‐I is a family of programmable nuclease systems that prefers a T‐rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome‐editing application of Cas12i3, a type V‐I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3‐based Multiplex direct repeats (DR)‐spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide‐resistant rice plants using the base editors. These CRIPSR/Cas12i3‐based genome editing systems will facilitate precision molecular breeding in plants.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>37822083</pmid><doi>10.1111/pbi.14192</doi><tpages>385</tpages><orcidid>https://orcid.org/0000-0003-3360-2799</orcidid><orcidid>https://orcid.org/0000-0002-4929-8317</orcidid><orcidid>https://orcid.org/0000-0001-7360-6837</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant biotechnology journal, 2024-02, Vol.22 (2), p.379-385 |
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| subjects | Arrays Base editors CRISPR CRISPR-Cas Systems - genetics CRISPR/Cas12i3 DNA structural variations Editing Efficiency Endonuclease Endonucleases - genetics Gene Editing - methods Gene expression Genome editing Genomes Herbicides Multiplex DR‐spacer Array Mutation Nuclease Oryza - genetics Plant breeding Plants - genetics Rice |
| title | Genome editing in rice using CRISPR/Cas12i3 |
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