Monolithic affinity columns in 3D printed microfluidics for chikungunya RNA detection

Mosquito-borne pathogens plague much of the world, yet rapid and simple diagnosis is not available for many affected patients. Using a custom stereolithography 3D printer, we created microfluidic devices with affinity monoliths that could retain, noncovalently attach a fluorescent tag, and detect ol...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2023-12, Vol.415 (29-30), p.7057-7065
Main Authors: Nielsen, Jacob B., Holladay, James D., Burningham, Addalyn J, Rapier-Sharman, Naomi, Ramsey, Joshua S., Skaggs, Timothy B., Nordin, Gregory P., Pickett, Brett E., Woolley, Adam T.
Format: Article
Language:English
Subjects:
3-D printers
Acids
Affinity
Analytical Chemistry
Animals
Aquatic insects
Biochemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chikungunya Fever - diagnosis
Chikungunya virus
Chikungunya virus - genetics
Chikungunya virus - metabolism
Dengue fever
Developing countries
Encephalitis
Fluorescence
Food Science
Genomes
Humans
Laboratory Medicine
LDCs
Lithography
Microfluidic devices
Microfluidics
Monitoring/Environmental Analysis
Mosquitoes
Oligonucleotides
Pathogens
Polymerase chain reaction
Printing, Three-Dimensional
Research Paper
Ribonucleic acid
RNA
RNA viruses
RNA, Viral - genetics
RNA, Viral - metabolism
Three dimensional printing
Vector-borne diseases
Viral infections
Viruses
Zika virus
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Description
Summary:Mosquito-borne pathogens plague much of the world, yet rapid and simple diagnosis is not available for many affected patients. Using a custom stereolithography 3D printer, we created microfluidic devices with affinity monoliths that could retain, noncovalently attach a fluorescent tag, and detect oligonucleotide and viral RNA. We optimized the fluorescent binding and sample load times using an oligonucleotide sequence from chikungunya virus (CHIKV). We also tested the specificity of CHIKV capture relative to genetically similar Sindbis virus. Moreover, viral RNA from both viruses was flowed through capture columns to study the efficiency and specificity of the column for viral CHIKV. We detected ~10 7 loaded viral genome copies, which was similar to levels in clinical samples during acute infection. These results show considerable promise for development of this platform into a rapid mosquito-borne viral pathogen detection system.
ISSN:1618-2642
1618-2650
1618-2650
DOI:10.1007/s00216-023-04971-6