Base J originally found in kinetoplastida is also a minor constituent of nuclear DNA of Euglena gracilis

We have analyzed DNA of EUGLENA: gracilis for the presence of the unusual minor base beta-D-glucosyl-hydroxymethyluracil or J, thus far only found in kinetoplastid flagellates and in DIPLONEMA: Using antibodies specific for J and post-labeling of DNA digests followed by two-dimensional thin-layer ch...

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Bibliographic Details
Published in:Nucleic acids research 2000-08, Vol.28 (16), p.3017-3021
Main Authors: Dooijes, D, Chaves, I, Kieft, R, Dirks-Mulder, A, Martin, W, Borst, P
Format: Article
Language:English
Subjects:
Animals
Base Sequence
beta -D-glucosyl-hydroxymethyluracil
Cell Nucleus - chemistry
Cell Nucleus - ultrastructure
Chromatography, Thin Layer
Diplonema
DNA Primers
DNA, Protozoan - chemistry
Euglena gracilis
Euglena gracilis - chemistry
Euglena gracilis - genetics
Glucosides - analysis
glucosyl-hydroxymethyluracil
Kinetoplastida
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Trypanosoma brucei brucei - chemistry
Trypanosoma brucei brucei - genetics
Uracil - analogs & derivatives
Uracil - analysis
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Summary:We have analyzed DNA of EUGLENA: gracilis for the presence of the unusual minor base beta-D-glucosyl-hydroxymethyluracil or J, thus far only found in kinetoplastid flagellates and in DIPLONEMA: Using antibodies specific for J and post-labeling of DNA digests followed by two-dimensional thin-layer chromatography of labeled nucleotides, we show that approximately 0.2 mole percent of EUGLENA: DNA consists of J, an amount similar to that found in DNA of Trypanosoma brucei. By staining permeabilized EUGLENA: cells with anti-J antibodies, we show that J is rather uniformly distributed in the EUGLENA: nucleus, and does not co-localize to a substantial extent with (GGGTTA)(n) repeats, the putative telomeric repeats of EUGLENA: Hence, most of J in EUGLENA: appears to be non-telomeric. Our results add to the existing evidence for a close phylogenetic relation between kinetoplastids and euglenids.
ISSN:1362-4962
0305-1048
1362-4962
DOI:10.1093/nar/28.16.3017