Identification of a bacterial inhibitor against g-type lysozyme

Lysozymes are antibacterial effectors of the innate immune system in animals that hydrolyze peptidoglycan. Bacteria have evolved protective mechanisms that contribute to lysozyme tolerance such as the production of lysozyme inhibitors, but only inhibitors of chicken (c-) and invertebrate (i-) type l...

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Published in:Cellular and molecular life sciences : CMLS 2011-03, Vol.68 (6), p.1053-1064
Main Authors: Vanderkelen, L, Van Herreweghe, J. M, Vanoirbeek, K. G. A, Baggerman, G, Myrnes, B, Declerck, P. J, Nilsen, I. W, Michiels, C. W, Callewaert, L
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aquatic birds
Bacteriology
Biochemistry
Biomedical and Life Sciences
Biomedicine
Biophysics
Cell Biology
Chromatography, Gel
DNA Primers - genetics
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Geese - metabolism
Goose-type lysozyme
Immune system
Immunological tolerance
Immunology
Inhibitors
Life Sciences
Lysozyme
Lysozyme inhibitor
Lysozyme tolerance
Mode of action
Molecular Sequence Data
Muramidase - antagonists & inhibitors
Muramidase - isolation & purification
Peptidoglycan
peptidoglycans
Polymers
Research Article
Surface Plasmon Resonance
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Summary:Lysozymes are antibacterial effectors of the innate immune system in animals that hydrolyze peptidoglycan. Bacteria have evolved protective mechanisms that contribute to lysozyme tolerance such as the production of lysozyme inhibitors, but only inhibitors of chicken (c-) and invertebrate (i-) type lysozyme have been identified. We here report the discovery of a novel Escherichia coli inhibitor specific for goose (g-) type lysozymes, which we designate PliG (periplasmic lysozyme inhibitor of g-type lysozyme). Although it does not inhibit c- or i-type lysozymes, PliG shares a structural sequence motif with the previously described PliI and MliC/PliC lysozyme inhibitor families, suggesting a common ancestry and mode of action. Deletion of pliG increased the sensitivity of E. coli to g-type lysozyme. The existence of inhibitors against all major types of animal lysozyme and their contribution to lysozyme tolerance suggest that lysozyme inhibitors may play a role in bacterial interactions with animal hosts.
ISSN:1420-682X
1420-9071
DOI:10.1007/s00018-010-0507-3