Evaluation of the histologic and immunohistochemical (CD34, glutamine synthetase) findings in idiopathic non-cirrhotic portal hypertension (INCPH)

Aim Idiopathic non-cirrhotic portal hypertension (INCPH) is a vascular disorder of uncertain origin. Diagnosis can be challenging on liver biopsy. Despite diverse histomorphologic findings documented in literature, studies on the frequency of these findings are lacking. This study aims to assess bot...

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Bibliographic Details
Published in:Hepatology international 2024-06, Vol.18 (3), p.1011-1019
Main Authors: Büyük, Melek, Berker, Neslihan, Bakkaloğlu, Doğu Vurallı, Şenkal, İbrahim Volkan, Önal, Zerrin, Güllüoğlu, Mine
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, CD34 - analysis
Antigens, CD34 - metabolism
Biopsy
Biopsy, Needle
CD34 antigen
Colorectal Surgery
Diagnosis
Evaluation
Female
Glutamate-ammonia ligase
Glutamate-Ammonia Ligase - analysis
Glutamate-Ammonia Ligase - metabolism
Glutamine
Hepatocytes
Hepatology
Humans
Hyperplasia
Hypertension
Hypertension, Portal - metabolism
Hypertension, Portal - pathology
Immunohistochemistry
Liver
Liver - pathology
Male
Medicine
Medicine & Public Health
Middle Aged
Original
Original Article
Portal vein
Portal Vein - pathology
Retrospective Studies
Staining
Surgery
Veins
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Summary:Aim Idiopathic non-cirrhotic portal hypertension (INCPH) is a vascular disorder of uncertain origin. Diagnosis can be challenging on liver biopsy. Despite diverse histomorphologic findings documented in literature, studies on the frequency of these findings are lacking. This study aims to assess both the histomorphologic features and the immunoexpression patterns of CD34 and glutamine synthetase (GS) in liver biopsies and searched for their contribution to the pathologic diagnosis of INCPH. Materials and methods Hematoxylin–eosin, CD34, and GS-stained liver needle biopsy sections of 16 patients clinically diagnosed with INCPH were retrospectively analyzed. Histologic findings such as portal vein narrowing, obliteration, or loss were grouped as major findings, while portal vein herniation, hypervascularized portal tracts, and periportal abnormal vessels were grouped as minor findings, and their frequency were evaluated. Periportal endothelial CD34 stained areas were measured via ocular micrometer. The distribution of GS immunoexpression was evaluated. Eighteen healthy liver donor biopsies were evaluated as controls. Results In INCPH cases, 58% of portal tracts showed major findings, compared to 15% in the control group (p 
ISSN:1936-0533
1936-0541
1936-0541
DOI:10.1007/s12072-024-10654-w