Polymorphism in the regulatory sequence of the human hsp70-1 gene does not affect heat shock factor binding or heat shock protein synthesis

A bi-allelic polymorphism found in the regulatory region of the human heat shock (HS) protein (HSP) hsp70-1 gene, which comprises an A-->C transversion, 3 bp upstream of the HS element (HSE), has been associated with extended HLA haplotypes. In view of the chaperoning and protective functions of...

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Bibliographic Details
Published in:Cellular and molecular life sciences : CMLS 1999-11, Vol.56 (7-8), p.701-708
Main Authors: Favatier, F, Jacquier-Sarlin, M R, Swierczewski, E, Polla, B S
Format: Article
Language:English
Subjects:
Alleles
Animals
B-Lymphocytes - metabolism
Base Sequence
Binding, Competitive
Cell Line, Transformed
Cellular biology
Conserved Sequence - genetics
DNA - genetics
DNA - metabolism
DNA-Binding Proteins - metabolism
Epstein-Barr virus
Genes
Haplotypes
Heat shock proteins
Heat Shock Transcription Factors
Heat-Shock Response - genetics
HLA Antigens - genetics
HSP70 Heat-Shock Proteins - biosynthesis
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Humans
Polymorphism
Polymorphism, Genetic - genetics
Promoter Regions, Genetic - genetics
Protein synthesis
Response Elements - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcription Factors
U937 Cells
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Summary:A bi-allelic polymorphism found in the regulatory region of the human heat shock (HS) protein (HSP) hsp70-1 gene, which comprises an A-->C transversion, 3 bp upstream of the HS element (HSE), has been associated with extended HLA haplotypes. In view of the chaperoning and protective functions of Hsp70, we investigated whether this hsp70-1 bi-allelic polymorphism could modulate the stress response, which may relate to enhanced resistance or susceptibility to certain diseases. We compared the basal and HS-induced HS factor (HSF)-binding activity of the two polymorphic HSEs, hsp70-1 mRNA accumulation and HSP expression in two human Epstein Barr virus (EBV)-transformed B cell lines typed for hsp70-1 promoter alleles. Our results suggest that hsp70-1 promoter polymorphism does not influence HSF-binding activity, hsp70 mRNA accumulation or synthesis in human EBV-transformed B cell lines.
ISSN:1420-682X
1420-9071
DOI:10.1007/s000180050463