The oral iron chelator deferasirox represses signaling through the mTOR in myeloid leukemia cells by enhancing expression of REDD1

• Published in: Cancer science 2009-05, Vol.100 (5), p.970-977
• Main Authors: Ohyashiki, Junko H., Kobayashi, Chiaki, Hamamura, Ryoko, Okabe, Seiichi, Tauchi, Tetsuzo, Ohyashiki, Kazuma
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Benzoates - administration & dosage; Benzoates - pharmacology; Biological and medical sciences; Cell Line, Tumor; Deferasirox; Disease Progression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - drug effects; Hematologic and hematopoietic diseases; Humans; Iron Chelating Agents - administration & dosage; Iron Chelating Agents - pharmacology; Leukemia, Myeloid - genetics; Leukemia, Myeloid - metabolism; Leukemia, Myeloid - pathology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; Mice; Mice, Nude; Original; Protein Kinases - metabolism; Ribosomal Protein S6 - metabolism; Signal Transduction - drug effects; TOR Serine-Threonine Kinases; Transcription Factors - genetics; Transcription Factors - metabolism; Triazoles - administration & dosage; Triazoles - pharmacology; Tumors; Up-Regulation; Xenograft Model Antitumor Assays
• ISSN: 1347-9032; 1349-7006; 1349-7006
• DOI: 10.1111/j.1349-7006.2009.01131.x

To evaluate the effect of deferasirox in human myeloid leukemia cells, and to identify the moleclular pathways responsible for antiproliferative effects on leukemia cells during chelation therapy, we performed gene expression profiling to focus on the pathway involved in the anticancer effect of deferasirox. The inhibitory concentration (IC50) of deferasirox was 17–50 µM in three human myeloid cell lines (K562, U937, and HL60), while those in fresh leukemia cells obtained from four patients it varied from 88 to 172 µM. Gene expression profiling using Affymerix GeneChips (U133 Plus 2.0) revealed up‐regulation of cyclin‐dependent kinase inhibitor 1A (CDKN1A) encoding p21CIP, genes regulating interferon (i.e. IFIT1). Pathways related to iron metabolism and hypoxia such as growth differentiation factor 15 (GDF‐15) and Regulated in development and DNA damage response (REDD1) were also prominent. Based on the results obtained from gene expression profiling, we particularly focused on the REDD1/mTOR (mammalian target of rapamycin) pathway in deferasirox‐treated K562 cells, and found an enhanced expression of REDD1 and its down‐stream protein, tuberin (TSC2). Notably, S6 ribosomal protein as well as phosphorylated S6, which is known to be a target of mTOR, was significantly repressed in deferasirox‐treated K562 cells, and REDD1 small interfering RNA restored phosphorylation of S6. Although iron chelation may affect multiple signaling pathways related to cell survival, our data support the conclusion that REDD1 functions up‐stream of tuberin to down‐regulate the mTOR pathway in response to deferasirox. Deferasirox might not only have benefit for iron chelation but also may be an antiproliferative agent in some myeloid leukemias, especially patients who need both iron chelation and reduction of leukemia cells. (Cancer Sci 2009; 100: 970–977)