Comparative analysis of endometrial, vaginal, and gut microbiota in patients with and without adenomyosis

• Published in: Acta obstetricia et gynecologica Scandinavica 2024-07, Vol.103 (7), p.1271-1282
• Main Authors: Valdés‐Bango, Marta, Gracia, Meritxell, Rubio, Elisa, Vergara, Andrea, Casals‐Pascual, Climent, Ros, Cristina, Rius, Mariona, Martínez‐Zamora, Maria Ángeles, Mension, Eduard, Quintas, Lara, Carmona, Francisco
• Format: Article
• Language: English
• Subjects: 16S rRNA sequencing; adenomyosis; Adenomyosis - microbiology; Adult; Case-Control Studies; Cross-Sectional Studies; Digestive system; endometrial microbiota; Endometriosis; Endometrium; Endometrium - microbiology; Female; Gastrointestinal Microbiome; gut microbiota; Gynecology; Humans; Microbiota; Middle Aged; Original; RNA, Ribosomal, 16S - genetics; Vagina; Vagina - microbiology; vaginal microbiota
• ISSN: 0001-6349; 1600-0412; 1600-0412
• DOI: 10.1111/aogs.14847

Introduction Alterations in microbiota composition have been implicated in a variety of human diseases. Patients with adenomyosis present immune dysregulation leading to a persistent chronic inflammatory response. In this context, the hypothesis that alterations in the microbiota may be involved in the pathogenesis of adenomyosis, by affecting the epigenetic, immunologic, and biochemical functions of the host, has recently been postulated. The aim of the present study was to compare the microbiota composition in the vagina, endometrium, and gut of individuals with and without adenomyosis. Material and Methods Cross‐sectional study including 38 adenomyosis patients and 46 controls, performed between September 2021 and October 2022 in a university hospital‐based research center. The diagnosis of adenomyosis was based on sonographic criteria. Fecal, vaginal, and endometrial samples were collected. Study of the microbiota using 16S rRNA gene sequencing. Results Patients with adenomyosis exhibited a significant reduction in the gut microbial alpha diversity compared with healthy controls (Chao1 p = 0.012, Fisher p = 0.005, Observed species p = 0.005). Beta‐diversity analysis showed significant differences in the compositions of both gut and vaginal microbiota between adenomyosis patients and the control group (Adonis p‐value = 0.001; R2 = 0.03 and Adonis p‐value = 0.034; R2 = 0.04 respectively). Specific bacterial taxa were found to be either overrepresented (Rhodospirillales, Ruminococcus gauvreauii group, Ruminococcaceae, and Actinomyces) or underrepresented in the gut and endometrial microbiota of adenomyosis patients compared with controls. Distinct microbiota profiles were identified among patients with internal and external adenomyosis phenotypes. Conclusions The study revealed reduced gut microbiota diversity in adenomyosis patients, accompanied by distinct compositions in gut and vaginal microbiota compared with controls. Overrepresented or underrepresented bacterial taxa were noted in the gut and endometrial microbiota of adenomyosis patients, with variations in microbiota profiles among those with internal and external adenomyosis phenotypes. These findings suggest a potential association between microbiota and adenomyosis, indicating the need for further research to comprehensively understand the implications of these differences. The study revealed reduced gut microbiota diversity in adenomyosis patients, accompanied by distinct compositions in gut