Comparative Anti-inflammatory Activity of a Nanocomposite-Based Herbal Oral Rinse and a Commercial Oral Rinse

• Published in: Curēus (Palo Alto, CA) CA), 2024-06, Vol.16 (6), p.e61548
• Main Authors: Divakar, Anjusha, Varghese, Remmiya Mary, S, Aravind Kumar, Shanmugam, Rajeshkumar
• Format: Article
• Language: English
• Subjects: Anti-inflammatory agents; Dentistry; Inflammation; Medical Education; Nanoparticles; Nanotechnology; Nitrates; Nonsteroidal anti-inflammatory drugs; Oral Medicine; Side effects; Silver; Sodium; Zinc oxides
• ISSN: 2168-8184; 2168-8184
• DOI: 10.7759/cureus.61548

The present study aimed to evaluate and compare the anti-inflammatory effects of two oral rinse formulations, a commercial oral rinse and an  and  (nanocomposites, NCs) oral rinse, using in vitro assays commonly employed to assess anti-inflammatory activity. The anti-inflammatory potential of the oral rinse formulations was assessed using bovine serum albumin (BSA) denaturation, egg albumin denaturation, and membrane stabilization assays. Diclofenac sodium was used as a reference standard in all assays. The inhibition percentages of BSA denaturation and egg albumin denaturation assays, as well as membrane stabilization effects, were measured at various concentrations of the oral rinse formulations. Both the commercial oral rinse and the  and  oral rinse demonstrated significant inhibition of BSA denaturation, indicating their anti-inflammatory potential. The  and (NCs) oral rinse consistently showed higher inhibition percentages than the commercial oral rinse, suggesting stronger anti-inflammatory effects in this assay. In the egg albumin denaturation assay, both formulations exhibited inhibition of protein denaturation, with the  and  (NCs) oral rinse showing comparable or slightly higher inhibition percentages. The membrane stabilization assay further supported the anti-inflammatory properties of both formulations, with the  and (NCs) oral rinse demonstrating efficacy comparable to diclofenac sodium. The results suggest that  and (NCs) oral rinse may possess stronger anti-inflammatory effects compared to commercial oral rinse, as evidenced by higher inhibition percentages in the BSA denaturation assay. Both formulations showed promising anti-inflammatory activity in the egg albumin denaturation and membrane stabilization assays, indicating their potential for mitigating inflammation. The  and (NCs) oral rinse exhibits significant anti-inflammatory effects in vitro, potentially surpassing the efficacy of the commercial oral rinse. Further studies are needed to explore the clinical implications of these findings and to validate the anti-inflammatory properties of the  and (NCs) oral rinse in vivo.