Non-interchangeable functions of efflux transporters of Pseudomonas aeruginosa in survival under infection-associated stress

• Published in: Journal of bacteriology 2024-07, Vol.206 (7), p.e0005424
• Main Authors: Adamiak, Justyna W, Ajmal, Laiba, Zgurskaya, Helen I
• Format: Article
• Language: English
• Subjects: Acids; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Bacteria; Bacterial diseases; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Bile; Bile salts; Defense mechanisms; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; Efflux; Gene Expression Regulation, Bacterial; Heat resistance; High temperature; Humans; Hydrogen-Ion Concentration; Iron; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Metabolites; Microbial Sensitivity Tests; Multidrug resistance; Nodulation; Opportunist infection; Osmotic Pressure; Osmotic stress; Pseudomonas aeruginosa; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - genetics; Pseudomonas aeruginosa - metabolism; Pseudomonas aeruginosa - physiology; Pseudomonas Infections - microbiology; Pumps; Research Article; Salts; Stress response; Stress, Physiological; Stresses; Substrates; Survival; Temperature; Toxins
• ISSN: 0021-9193; 1098-5530; 1098-5530
• DOI: 10.1128/jb.00054-24

is a challenging opportunistic pathogen due to its intrinsic and acquired mechanisms of antibiotic resistance. A large repertoire of efflux transporters actively expels antibiotics, toxins, and metabolites from cells and enables growth of in diverse environments. In this study, we analyzed the roles of representative efflux pumps from the Resistance-Nodulation-Division (RND), Major Facilitator Superfamily (MFS), and Small Multidrug Resistance (SMR) families of proteins in the susceptibility of to antibiotics and bacterial growth under stresses imposed by human hosts during bacterial infections: an elevated temperature, osmotic stress, low iron, bile salts, and acidic pH. We selected five RND pumps MexAB-OprM, MexEF-OprN, MexCD-OprJ, MuxABC-OpmB, and TriABC-OpmH that differ in their substrate specificities and expression profiles, two MFS efflux pumps PA3136-3137 and PA5158-5160 renamed here into MfsAB and MfsCD-OpmG, respectively, and an SMR efflux transporter PA1540-1541 (MdtJI). We found that the most promiscuous RND pumps such as MexEF-OprN and MexAB-OprM are integrated into diverse survival mechanisms and enable growth under various stresses. MuxABC-OpmB and TriABC-OpmH pumps with narrower substrate spectra are beneficial only in the presence of the iron chelator 2,2'-dipyridyl and bile salts, respectively. MFS pumps do not contribute to antibiotic efflux but play orthogonal roles in acidic pH, low iron, and in the presence of bile salts. In contrast, MdtJI protects against polycationic antibiotics but does not contribute to survival under stress. Thus, efflux pumps play specific, non-interchangeable functions in cell physiology and bacterial survival under stresses. The role of multidrug efflux pumps in the intrinsic and clinical levels of antibiotic resistance in and other gram-negative bacteria is well-established. Their functions in bacterial physiology, however, remain unclear. The genome comprises an arsenal of efflux pumps from different protein families, the substrate specificities of which are typically assessed by measuring their impact on susceptibility to antibiotics. In this study, we analyzed how deletions and overproductions of efflux pumps affect growth under human-infection-induced stresses. Our results show that the physiological functions of multidrug efflux pumps are non-redundant and essential for the survival of this important human pathogen under stress.