Advances in submerged liquid fermentation and formulation of entomopathogenic fungi

• Published in: Applied microbiology and biotechnology 2024-12, Vol.108 (1), p.451, Article 451
• Main Authors: Mascarin, Gabriel Moura, Golo, Patrícia Silva, de Souza Ribeiro-Silva, Cárita, Muniz, Elen Regozino, de Oliveira Franco, Artur, Kobori, Nilce Naomi, Fernandes, Éverton Kort Kamp
• Format: Article
• Language: English
• Subjects: Animals; Biological control; Biological Control Agents - metabolism; Biomedical and Life Sciences; Biotechnology; Bright plating; Chlamydospores; Commercialization; Conidia; Dietary supplements; Effectiveness; Entomopathogenic fungi; Environmental factors; Fermentation; Fungi; Fungi - metabolism; Insecta - microbiology; Life Sciences; Mass production; Microbial Genetics and Genomics; Microbiology; Microorganisms; Mini-Review; Molecular modelling; Pest control; Pest Control, Biological - methods; Pests; Production methods; Propagules; Robust control; Shelf life; Storage stability; Substrates; Vectors
• ISSN: 0175-7598; 1432-0614; 1432-0614
• DOI: 10.1007/s00253-024-13287-z

Entomopathogenic fungi (EPF) can be defined as beneficial multifunctional eukaryotic microorganisms that display pivotal ecological services in pest management, with some species possessing the special ability to establish mutualistic relationships with plants. Mass production of these fungi is critical to support affordable widespread commercialization and worldwide field application. Among the mass production methods explored mainly by industry, submerged liquid fermentation is a robust and versatile technology that allows the formation of different types of propagules designated for various applications in pest control. Many hypocrealean EPF are easily culturable on artificial substrates by producing single-celled structures (hyphal bodies, blastospores, and submerged conidia) or multicellular structures (mycelium and microsclerotia). Less frequently, some EPF may form environmentally resistant chlamydospores, but these structures have almost always been overlooked. A continued research pipeline encompassing screening fungal strains, media optimization, and proper formulation techniques aligned with the understanding of molecular cues involved in the formation and storage stability of these propagules is imperative to unlock the full potential and to fine-tune the development of robust and effective biocontrol agents against arthropod pests and vectors of diseases. Finally, we envision a bright future for the submerged liquid fermentation technology to supplement or replace the traditional solid substrate fermentation method for the mass production of many important EPF. Key points • Submerged liquid fermentation (SLF) allows precise control of nutritional and environmental factors • SLF provides a scalable, robust, and cost-effective platform for mycopesticide production • Enhancing formulation, shelf life, and field efficacy of submerged propagules remain crucial • Understanding the molecular mechanisms behind submerged propagule formation is key to advancing SLF technology Graphical abstract