Longitudinal associations between urinary biomarkers of phthalates and replacements with novel in vivo measures of placental health

• Published in: Human reproduction (Oxford) 2024-09, Vol.39 (9), p.2104-2114
• Main Authors: Rosen, Emma M, Stevens, Danielle R, McNell, Erin E, Wood, Mollie E, Engel, Stephanie M, Keil, Alexander P, Calafat, Antonia M, Botelho, Julianne Cook, Sinkovskaya, Elena, Przybylska, Ann, Saade, George, Abuhamad, Alfred, Ferguson, Kelly K
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers - urine; Calcinosis - chemically induced; Calcinosis - diagnostic imaging; Calcinosis - urine; Female; Humans; Longitudinal Studies; Maternal Exposure - adverse effects; Microvessels - diagnostic imaging; Microvessels - drug effects; Original; Phthalic Acids - urine; Placenta - diagnostic imaging; Placenta - metabolism; Pregnancy; Prospective Studies; Ultrasonography, Prenatal; Young Adult
• ISSN: 0268-1161; 1460-2350; 1460-2350
• DOI: 10.1093/humrep/deae152

Abstract STUDY QUESTION What is the longitudinal association between gestational phthalate exposure and in vivo placental outcomes? SUMMARY ANSWER Phthalates were adversely associated with placental microvasculature, stiffness, and presence of calcification, with different metabolites associated with different outcomes. WHAT IS KNOWN ALREADY Phthalate exposure is ubiquitous and implicated as a contributor to adverse pregnancy outcomes, possibly through impacts on the placenta. STUDY DESIGN, SIZE, DURATION A total of 303 women were recruited in early pregnancy and prospectively followed for up to eight visits across gestation in the Human Placenta and Phthalates study. PARTICIPANTS/MATERIALS, SETTING, METHODS At each visit, women provided urine samples and underwent placental ultrasounds. Urine was analyzed for 18 metabolites of phthalates and replacements. We took the geometric mean of repeated measurements to reflect pregnancy-averaged phthalate or replacement exposure for each participant (n = 303). Placental microvasculature, stiffness, and microcalcification presence were quantified from ultrasounds at each visit. Higher scores reflected worse placental function for all measures. Generalized linear mixed models were created to estimate the association between pregnancy-averaged exposure biomarker concentrations and repeated outcome measurements for microvasculature and stiffness. Gestational age at the time of calcification detection was modeled using Cox proportional hazards models. MAIN RESULTS AND THE ROLE OF CHANCE Monocarboxyisononyl phthalate and summed di(2-ethylhexyl) phthalate metabolites were associated with impaired microvasculature development, such that an interquartile range increase in concentration was associated with 0.11 standard deviation increase in the microvasculature ratio, indicating poorer vascularization (95% CI: 0.00, 0.22); 0.11 [95% CI: −0.01, 0.22], respectively. Monoethyl phthalate was associated with increased placental stiffness (0.09 [95% CI: −0.01, 0.19]) while summed di-iso-butyl phthalate metabolites and monobenzyl phthalate were associated with increased hazard of calcification detection (hazard ratios: 1.18 [95% CI: 0.98, 1.42]; 1.13 [95% CI: 0.96, 1.34]). LIMITATIONS, REASONS FOR CAUTION Outcomes used in this study are novel and further investigation is needed to provide clinical context and relevance. WIDER IMPLICATIONS OF THE FINDINGS We found evidence of associations between select phthalate biomarkers and var