Detection of cytomegalovirus by in situ hybridisation and immunohistochemistry using new monoclonal antibody CCH2: a comparison of methods

In situ hybridisation, immunohistochemistry, and morphological analysis for the detection of cytomegalovirus (CMV) were compared in routinely processed tissue sections from a patient with acquired immune deficiency syndrome (AIDS) and widespread CMV infection. Both in situ hybridisation and immunohi...

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Bibliographic Details
Published in:Journal of clinical pathology 1988-09, Vol.41 (9), p.1005-1009
Main Authors: Niedobitek, G, Finn, T, Herbst, H, Gerdes, J, Grillner, L, Landqvist, M, Wirgart, B Z, Stein, H
Format: Article
Language:English
Subjects:
Acquired Immunodeficiency Syndrome - complications
Acquired Immunodeficiency Syndrome - microbiology
Adult
AIDS/HIV
Antibodies, Monoclonal - immunology
Antibodies, Viral - immunology
Biological and medical sciences
Cytomegalovirus - isolation & purification
Cytomegalovirus Infections - complications
DNA, Viral
Humans
Immunohistochemistry
Infectious diseases
Male
Medical sciences
Nucleic Acid Hybridization
Viral diseases
Virology - methods
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Summary:In situ hybridisation, immunohistochemistry, and morphological analysis for the detection of cytomegalovirus (CMV) were compared in routinely processed tissue sections from a patient with acquired immune deficiency syndrome (AIDS) and widespread CMV infection. Both in situ hybridisation and immunohistochemistry with the monoclonal antibody CCH2 labelled all "owl's eye" cells intensely and, in addition, nuclei of some morphologically normal cells. Quantitative evaluation of the results showed that in situ hybridisation and immunohistochemistry with CCH2 were considerably more sensitive than purely morphological analysis, particularly in tissues with only a few cells infected by CMV. It is further shown that immunohistochemistry with CCH2 detected a higher figure of CMV infected cells than in situ hybridisation. In conclusion, both in situ hybridisation and immunohistochemistry are rapid, sensitive, and specific methods for CMV detection. For routine purposes, however, immunohistochemistry seems to be more suitable.
ISSN:0021-9746
1472-4146
DOI:10.1136/jcp.41.9.1005