Microinjected glutathione S-transferase Yb subunits translocate to the cell nucleus

We have previously shown that a 30 kDa DNA-binding protein isolated from rat cell nuclei exhibits the chemical and immunological properties of glutathione S-transferase Yb subunits [Bennett, Spector & Yeoman (1986) J. Cell Biol. 102, 600-609]. It was of interest, therefore, to determine whether...

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Bibliographic Details
Published in:Biochemical journal 1987-10, Vol.247 (1), p.109-112
Main Authors: Bennett, C F, Yeoman, L C
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - enzymology
Cells, Cultured
Glutathione Transferase - metabolism
Isoenzymes - metabolism
Microinjections
Rats
Subcellular Fractions - enzymology
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Summary:We have previously shown that a 30 kDa DNA-binding protein isolated from rat cell nuclei exhibits the chemical and immunological properties of glutathione S-transferase Yb subunits [Bennett, Spector & Yeoman (1986) J. Cell Biol. 102, 600-609]. It was of interest, therefore, to determine whether Yb subunits isolated from rat liver nuclei would return to nuclear fractions upon reintroduction to cell cytoplasms via red-blood-cell-mediated fusion. Labelled Yb subunits were associated with nuclear fractions 60 min after cell fusion. The microinjected protein remained associated with the nuclei for 18 h and was not extractable with low-salt washes. In addition, injected Yb subunits were found to equally distribute between extractable (56%) and residual (44%) nuclear fractions. These experiments demonstrate that glutathione S-transferase Yb subunits isolated from nuclei rapidly translocate to nuclei upon reintroduction into cell cytoplasms.
ISSN:0264-6021
1470-8728
DOI:10.1042/bj2470109