Distinct brain and lung endothelial miRNA/mRNA profiles after exposure to Plasmodium falciparum-infected red blood cells

MicroRNAs (miRNAs) control 60% of genes expressed in the human body, but their role in malaria pathogenesis is incompletely understood. Here, we demonstrate cell type-specific alterations to the miRNA profiles during the early response to malaria infection in brain and lung endothelial cells (ECs)....

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Published in:iScience 2024-11, Vol.27 (11), p.111265, Article 111265
Main Authors: Metwally, Nahla Galal, Tauler, Maria del Pilar Martinez, Torabi, Hanifeh, Allweier, Johannes, Mohamed, Sara, Bessemoulin, Maryeva, Bouws, Philip, Alshikh, Fatima, Wu, Yifan, Temori, Milad, Schell, Tabea, Rakotonirinalalao, Maximillian, Honecker, Barbara, Höhn, Katharina, Jacobs, Thomas, Heine, Holger, Bruchhaus, Iris
Format: Article
Language:English
Subjects:
Biological sciences
Disease
Microbiology parasite
Neuroscience
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Summary:MicroRNAs (miRNAs) control 60% of genes expressed in the human body, but their role in malaria pathogenesis is incompletely understood. Here, we demonstrate cell type-specific alterations to the miRNA profiles during the early response to malaria infection in brain and lung endothelial cells (ECs). In brain ECs, incubation with Plasmodium falciparum-infected red blood cells in the ring stage (iRBCs) most significantly affected endocytosis-related miRNAs and mRNAs. Contrastingly, in lung ECs, iRBCs altered electron transport chain-related miRNAs and mRNAs. We present a dataset of inherent differences between microRNA profiles in brain and lung ECs and their extracellular vesicles (EVs). We demonstrated that shear stress affected multiple pathways in brain ECs, which were controlled by numerous human miRNAs. Together, these findings indicate that host miRNAs respond to parasite exposure, accompanied by stimulation of downstream signaling pathways within the ECs. Therefore, we consider miRNAs the initial spark for early host-parasite interaction events. [Display omitted] •Shear stress reinforced tight junctions in brain endothelium•Endocytic pathways were activated in the brain endothelium•The electron transport chain pathway was activated in lung endothelium•miRNA candidates might be causing the mRNA alterations within the endothelium Disease; Biological sciences; Neuroscience; Microbiology parasite
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2024.111265