Swill and Pig Manure Substrates Differentially Affected Transcriptome and Metabolome of the Black Soldier Fly Larvae

• Published in: International journal of molecular sciences 2024-11, Vol.25 (22), p.12147
• Main Authors: Zhang, Bin, Yang, Rencan, He, Shichun, Dai, Sifan, Hu, Qingquan, Li, Xinrong, Su, Hongren, Shi, Jingyi, Zhao, Zhiyong, Wu, Dongwang
• Format: Article
• Language: English
• Subjects: Amino acids; Analysis; Animals; Antibiotics; Biomass; Biosynthesis; China; Climate change; Diptera - genetics; Diptera - growth & development; Diptera - metabolism; Energy Metabolism - genetics; Fatty acids; Feeds; Gene expression; Gene Expression Profiling - methods; Genes; Genetic aspects; Larva - genetics; Larva - growth & development; Larva - metabolism; Manure; Manures; Massachusetts; Metabolism; Metabolites; Metabolome; Natural resources; Nitrogen; Organic waste treatment; Physiology; Proteins; Refuse and refuse disposal; Soldiers; Swine; Transcriptome; Waste management
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms252212147

Black soldier fly larvae ( ) (BSFL) are insect larvae with significant ecological and economic value. This study aims to investigate whether swill and manure had any effects on the transcriptome and metabolome of BSFL. Through high-throughput transcriptome sequencing, we found that larvae fed with swill exhibited higher levels of gene expression, especially with the upregulation of genes related to energy metabolism, amino acid metabolism, and redox reactions. Metabolomics analysis showed a significant increase in energy metabolism-related metabolites, such as organic acids and amino acids, in the swill-fed larvae. In contrast, gene expression and metabolic characteristics in the pig manure-fed group indicated a higher stress response, with relevant genes and metabolites (such as short-chain fatty acids and antioxidants) showing significant upregulation. GO and KEGG enrichment analyses further supported these results, suggesting that swill promotes larval growth and metabolism, whereas pig manure induces the activation of stress response mechanisms. These findings offer clear molecular and physiological insights into the optimization of substrate selection for enhancing the performance of BSFL in waste management.