Single‐Cell RNA Sequencing Analysis Reveals Exercise‐Induced Transcriptional Dynamics in Half‐Marathon Runners

ABSTRACT Previous studies in sports science suggested that regular exercise has a positive impact on human health. However, the effects of endurance sports and their underlying mechanisms are still not completely understood. One of the main debates regards the modulation of immune dynamics in high‐i...

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Published in:Scandinavian journal of medicine & science in sports 2025-01, Vol.35 (1), p.e70018-n/a
Main Authors: Veschetti, Laura, Patuzzo, Cristina, Treccani, Mirko, Moron Dalla Tor, Lucas, Deiana, Michela, Cheri, Samuele, Griggio, Francesca, Lippi, Giuseppe, Schena, Federico, De Santis, Daniele, Dalle Carbonare, Luca, Tarperi, Cantor, Trabetti, Elisabetta, Valenti, Maria Teresa, Malerba, Giovanni
Format: Article
Language:English
Subjects:
Adult
Exercise
Female
Humans
immune modulation
Leukocytes, Mononuclear - metabolism
Male
Marathon Running - physiology
Marathons
Middle Aged
Original
Physical Endurance - physiology
Running
Sequence Analysis, RNA
Single-Cell Analysis
single‐cell RNA sequencing
Transcriptome
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Summary:ABSTRACT Previous studies in sports science suggested that regular exercise has a positive impact on human health. However, the effects of endurance sports and their underlying mechanisms are still not completely understood. One of the main debates regards the modulation of immune dynamics in high‐intensity exercise. As part of the “Run 4 Science” project in Verona, Italy, we conducted a single‐cell RNA sequencing analysis on half‐marathon amateur runners to investigate the transcriptional dynamics of peripheral blood mononuclear cells following endurance exercise. Blood samples were collected from four participants before and after running a half‐marathon to carry out a comprehensive transcriptomic analysis of immune cells at the single‐cell level. Our analysis revealed significant alterations in the transcriptional profiles following endurance physical exercise. Modulations in myeloid cells suggested the activation of stress response (6 related pathways, p 
ISSN:0905-7188
1600-0838
1600-0838
DOI:10.1111/sms.70018