Purification and partial characterization of a cadmium-binding protein from the liver of rainbow trout (Onchorynchus mykiss)

This study describes the isolation and partial characterization of a low molecular weight (approximately 14 kDa), cadmium-binding protein from rainbow trout (Onchorynchus mykiss) liver. Rainbow trout were injected intraperitoneally with 3.5 mg/kg cadmium chloride (total body dose) twice weekly for 3...

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Bibliographic Details
Published in:Canadian journal of veterinary research 1999-10, Vol.63 (4), p.225-229
Main Authors: Mullins, J.E, Fredrickson, R.A, Fuentealba, I.C, Markham, R.J.F
Format: Article
Language:English
Subjects:
amino acids
Animals
Antibodies, Monoclonal
binding proteins
cadmium
Cadmium - toxicity
heavy metals
induction
liver
Liver - chemistry
metallothionein
Metallothionein - isolation & purification
Mice
monoclonal antibodies
Oncorhynchus mykiss
Oncorhynchus mykiss - physiology
poisoning
testes
Water Pollutants, Chemical - toxicity
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Summary:This study describes the isolation and partial characterization of a low molecular weight (approximately 14 kDa), cadmium-binding protein from rainbow trout (Onchorynchus mykiss) liver. Rainbow trout were injected intraperitoneally with 3.5 mg/kg cadmium chloride (total body dose) twice weekly for 3 wk. Livers were removed and a cadmium-binding protein was isolated. Monoclonal antibodies produced against this protein were used in the affinity purification process. Amino acid analysis showed the protein contained 3.8 mol% cysteine, 3.5 mol% phenylalanine, 2.2 mol% tyrosine and 1.9 mol% histidine. The low cysteine content suggests that it was distinct from metallothionein. The monoclonal antibodies were also used to identify the protein in liver homogenates from both cadmium-exposed and control fish and in the testes of cadmium-exposed mice lacking the gene for both metallothionein-1 and metallothionein-II. The compound identified in this study represents a non-metallothionein cadmium-binding protein that appears to be highly conserved.
ISSN:0830-9000