Rearrangements involving repeated sequences within a P element preferentially occur between units close to the transposon extremities

• Published in: Genetics (Austin) 1996-02, Vol.142 (2), p.459-470
• Main Authors: Paques, F, Bucheton, B, Wegnez, M
• Format: Article
• Language: English
• Subjects: Animals; ATP-Binding Cassette Transporters; color; compound eyes; deletions; dna amplification; DNA Transposable Elements; Drosophila melanogaster; Drosophila Proteins; duplication; Eye Color - genetics; Eye Proteins; Female; Gene Frequency; Gene Rearrangement; genetic change; genetic markers; genetic recombination; Genetics; Insect Hormones - genetics; Insects; Investigations; Male; Multigene Family; Mutagenesis, Insertional; Phenotype; repetitive sequences; Repetitive Sequences, Nucleic Acid; ribosomal DNA; Transformation, Genetic; transposition; transposons; white gene
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/142.2.459

In a previous report we described rearrangements occurring at a high rate (30% of the progeny of dysgenic flies) within a cluster of 5S genes internal to a P element. These events were characterized as precise amplifications and deletions of 5S units. Here we analyze recombination events within P elements containing two repeated arrays of 5S genes flanking a central white gene. Deletions (50%) and duplications (3%) of the white gene together with various amounts of flanking 5S genes were observed. These recombinations occur preferentially between the most external 5S units of P transposons. Such rearrangements could be favored by interactions between the proteins bound to the P terminal sequences.