Molecular cloning and immunolocalization of a novel vertebrate trp homologue from Xenopus

We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two p...

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Bibliographic Details
Published in:Biochemical journal 1999-06, Vol.340 ( Pt 3) (3), p.593-599
Main Authors: Bobanovic, L K, Laine, M, Petersen, C C, Bennett, D L, Berridge, M J, Lipp, P, Ripley, S J, Bootman, M D
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Base Sequence
Calmodulin-Binding Proteins - analysis
Calmodulin-Binding Proteins - chemistry
Calmodulin-Binding Proteins - genetics
Cell Membrane - chemistry
Cloning, Molecular
Drosophila Proteins
Freshwater
Gene Library
HeLa Cells
Humans
Immunohistochemistry
Membrane Proteins - analysis
Membrane Proteins - chemistry
Membrane Proteins - genetics
Molecular Sequence Data
Molecular Weight
Oocytes - cytology
Oocytes - metabolism
Sequence Alignment
Sequence Homology, Amino Acid
Transient Receptor Potential Channels
trp gene
trp1 gene
Xenopus
Xenopus laevis - genetics
Xtrp gene
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Summary:We report the sequence, structure and distribution of a novel transient receptor potential (trp) homologue from Xenopus, Xtrp, determined by screening an oocyte cDNA library. On the basis of sequence similarity and predicted structure, Xtrp appears to be a homologue of mammalian trp1 proteins. Two polyclonal antibodies raised against distinct regions of the Xtrp sequence revealed Xtrp expression in various Xenopus tissues, and the localization of Xtrp at the plasma membrane of Xenopus oocytes and HeLa cells. Since capacitative calcium entry into Xenopus oocytes has been shown previously to be substantially inhibited by trp1 antisense oligonucleotides [Tomita, Kaneko, Funayama, Kondo, Satoh and Akaike (1998) Neurosci. Lett. 248, 195-198] we suggest that Xtrp may underlie capacitative calcium entry in Xenopus tissues.
ISSN:0264-6021
1470-8728
DOI:10.1042/0264-6021:3400593