Induction of pulmonary antibodies to Pasteurella haemolytica following intraduodenal stimulation of the gut-associated lymphatic tissue in cattle

The induction of pulmonary antibodies to a bacterial antigen following intraduodenal (D) stimulation of the gut-associated lymphatic tissue (GALT) was investigated. Six calves were divided into two groups of three calves each. The GALT-primed calves received an ID dose of live Pasteurella haemolytic...

Full description

Saved in:
Bibliographic Details
Published in:Canadian journal of veterinary research 1989-10, Vol.53 (4), p.371-377
Main Authors: Bowersock, T.L, Walker, R.D, McCracken, M.D, Hopkins, F.M, Moore, R.N
Format: Article
Language:English
Subjects:
Animals
antibodies
Antibodies, Bacterial - biosynthesis
antibody formation
bacterial antigens
Bacterial Toxins - immunology
Cattle
Cattle Diseases - immunology
duodenum
Exotoxins - immunology
immunoglobulin A
Immunoglobulin A - biosynthesis
immunoglobulin G
Immunoglobulin G - biosynthesis
Intestinal Mucosa - immunology
Lung - immunology
Lung - pathology
lymphatic system
Lymphoid Tissue - immunology
Mannheimia haemolytica
Pasteurella - immunology
Pasteurella Infections - immunology
Pasteurella Infections - veterinary
pasteurellosis
Pneumonia - immunology
Pneumonia - veterinary
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The induction of pulmonary antibodies to a bacterial antigen following intraduodenal (D) stimulation of the gut-associated lymphatic tissue (GALT) was investigated. Six calves were divided into two groups of three calves each. The GALT-primed calves received an ID dose of live Pasteurella haemolytica A1 followed by a subcutaneous (SC) dose of killed P. haemolytica. The sham-primed calves received an ID dose of phosphate-buffered saline solution (PBSS) followed by a SC dose of killed bacteria. Serum and pulmonary lavage fluids were collected weekly from each calf and assayed for titers of leukotoxin neutralizing antibodies (LNA), as well as IgG and IgA (lavage fluids only) to P. haemolytica. The GALT-primed calves responded to the ID stimulation by bacteria with increased serum IgG. The sham-primed calves had no change in antibody titers following ID stimulation. The GALT-primed calves had increased serum IgG, lavage IgG and IgA and increased LNA titers in both lavage fluids and serum following the SC dose of killed bacteria. The sham-primed calves demonstrated only an increase in serum IgG following the SC inoculation. A challenge study to evaluate if antibodies induced by GALT stimulation could reduce pulmonary lesions was performed using six calves divided into two groups. One group received an ID dose of P. haemolytica followed two weeks later by a SC dose of killed P. haemolytica. The sham vaccinated calves received an ID dose of PBSS followed in two weeks by a SC dose of killed bacterin. Calves were challenged by an intrapulmonary dose of live P. haemolytica A1 eleven days after the SC inoculation.
ISSN:0830-9000