Epsilon-tubulin is an essential component of the centriole

Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement an...

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Bibliographic Details
Published in:Molecular biology of the cell 2002-11, Vol.13 (11), p.3859-3869
Main Authors: Dutcher, Susan K, Morrissette, Naomi S, Preble, Andrea M, Rackley, Craig, Stanga, John
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Centrioles - metabolism
Chlamydomonas - cytology
Chlamydomonas - physiology
Humans
Microtubules - metabolism
Molecular Sequence Data
Phenotype
Phylogeny
Protein Isoforms - classification
Protein Isoforms - genetics
Protein Isoforms - metabolism
Protozoan Proteins - chemistry
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Sequence Alignment
Tubulin - chemistry
Tubulin - classification
Tubulin - genetics
Tubulin - metabolism
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Summary:Centrioles and basal bodies are cylinders composed of nine triplet microtubule blades that play essential roles in the centrosome and in flagellar assembly. Chlamydomonas cells with the bld2-1 mutation fail to assemble doublet and triplet microtubules and have defects in cleavage furrow placement and meiosis. Using positional cloning, we have walked 720 kb and identified a 13.2-kb fragment that contains epsilon-tubulin and rescues the Bld2 defects. The bld2-1 allele has a premature stop codon and intragenic revertants replace the stop codon with glutamine, glutamate, or lysine. Polyclonal antibodies to epsilon-tubulin show peripheral labeling of full-length basal bodies and centrioles. Thus, epsilon-tubulin is encoded by the BLD2 allele and epsilon-tubulin plays a role in basal body/centriole morphogenesis.
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.E02-04-0205