Ordered differential display: a simple method for systematic comparison of gene expression profiles

A method for display of 3′-end restriction fragments of cDNAs is proposed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression effect. Then, simplified subsets of these fragments suitable for comparison by PAGE are ampli...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1997-06, Vol.25 (12), p.2541-2542
Main Authors: Matz, Mikhail, Usman, Natalia, Shagin, Dmitryi, Bogdanova, Ekaterina, Lukyanov, Sergey
Format: Article
Language:English
Subjects:
Animals
Base Sequence
DNA Primers
DNA, Complementary
DNA, Helminth - biosynthesis
DNA, Helminth - chemistry
Dugesia tigrina
Gene Expression
Planarians - genetics
Planarians - metabolism
Polymerase Chain Reaction - methods
Restriction Mapping - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for display of 3′-end restriction fragments of cDNAs is proposed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression effect. Then, simplified subsets of these fragments suitable for comparison by PAGE are amplified by adapter-specific primers extended by two randomly picked bases at their 3′ ends. By testing all possible combinations of extended primers the whole mRNA pool may be systematically investigated. The method was applied to search for molecular regional markers of freshwater planarian Dugesia tigrina.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/25.12.2541