Chromosome Painting in Biological Dosimetry: Assessment of the Ability to Score Stable Chromosome Aberrations Using Different Pairs of Paint Probes

We exposed human peripheral lymphocytes in vitro to 0.3 and 1 Gy of60Co gamma rays to evaluate whether the ability and sensitivity to detect chromosomal aberrations by chromosome painting is independent or not to the specific paint probes. To detect structural aberrations (translocations), we painte...

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Bibliographic Details
Published in:Environmental health perspectives 1996-05, Vol.104 (Suppl 3), p.475-477
Main Authors: García-Sagredo, Jose M., Vallcorba, Isabel, López-Yarto, Ana, Maria Del Carmen Sanchez-Hombre, Resino, Monica, Ferro, Maria Teresa
Format: Article
Language:English
Subjects:
Adult
Biomarkers of Exposure and Effects of Mutagens and Carcinogens
Chromosome Aberrations
Chromosome painting
Chromosome translocation
Chromosomes
Cobalt Radioisotopes
DNA Probes
Dose-Response Relationship, Radiation
Dosimetry
Fluorescence in situ hybridization
Gamma Rays
Genomes
Humans
In Situ Hybridization, Fluorescence
In Vitro Techniques
Lymphocytes - radiation effects
Male
Metaphase
Radiation dosage
Staining and Labeling
Translocation, Genetic - radiation effects
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Summary:We exposed human peripheral lymphocytes in vitro to 0.3 and 1 Gy of60Co gamma rays to evaluate whether the ability and sensitivity to detect chromosomal aberrations by chromosome painting is independent or not to the specific paint probes. To detect structural aberrations (translocations), we painted chromosome spreads simultaneously with two whole-chromosome libraries for chromosomes 1, 2, 3, 4, 5, 6, 7, 11, 13, 16, and 18. To compare the rate of chromosome translocations detected by the different pairs of chromosomes, data were normalized according to the fraction of genome painted and evaluated by unconditional logistic regression. Our results show that any combination of paint probes can be used to score induced chromosomal aberrations. We observed that the amounts of translocations are dose dependent and quite homogeneous within each dose of radiation, independently of chromosomes painted. However, the use of small chromosome probes is not recommended because of the high number of cells to be analyzed due to the small amount of genome painted and because it is more difficult to detect translocations in small chromosomes.
ISSN:0091-6765
DOI:10.2307/3432807