Saccharomyces cerevisiae mms19 mutants are deficient in transcription-coupled and global nucleotide excision repair

The recently cloned Saccharomyces cerevisiae MMS19 gene appears to be involved in both nucleotide excision repair (NER) and transcription, which is also the case for components of the NER/transcription complex TFIIH. Unlike TFIIH however, the Mms19 protein does not affect NER in a highly purified in...

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Bibliographic Details
Published in:Nucleic acids research 1997-10, Vol.25 (20), p.3974-3979
Main Authors: Lombaerts, M, Tijsterman, M, Verhage, R.A, Brouwer, J
Format: Article
Language:English
Subjects:
biological resistance
cyclobutane pyrimidine dimers
dna modification
DNA repair
DNA Repair - genetics
DNA, Fungal - metabolism
Fungal Proteins - genetics
global genome repair
Kinetics
mutants
Mutation
Nucleotides - metabolism
phenotype
Polymerase Chain Reaction
Pyrimidine Dimers - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
transcription (genetics)
Transcription Factors
Transcription, Genetic - genetics
ultraviolet radiation
Ultraviolet Rays
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Summary:The recently cloned Saccharomyces cerevisiae MMS19 gene appears to be involved in both nucleotide excision repair (NER) and transcription, which is also the case for components of the NER/transcription complex TFIIH. Unlike TFIIH however, the Mms19 protein does not affect NER in a highly purified in vitro system. In order to investigate the role of Mms19 in NER, we have analysed the repair capacity of the mms19 disruption mutant. We find that a cell-free extract of this mutant is deficient for NER in vitro. Since mms19 mutants are only moderately sensitive to irradiation with ultraviolet (UV) light, it is possible that such mutants are specifically deficient in one of the two modes of NER, i.e. transcription-coupled or global genome repair. To investigate this possibility, we have analysed the removal of cyclobutane-pyrimidine dimers (CPDs) at the nucleotide level in an mms19 mutant. Repair of CPDs was not detectable for both transcribed and non-transcribed sequences in this mutant, demonstrating a requirement for Mms19 in both transcription-coupled and global genome repair. Our data, combined with those obtained by others, suggest that Mms19 is required for NER in yeast, although it seems likely that the protein plays an indirect role in this process.
ISSN:0305-1048
1362-4962
1362-4962
DOI:10.1093/nar/25.20.3974