Analysis of the spontaneous in vitro anti‐HIV‐1 antibody secretion by peripheral blood mononuclear cells in HIV‐1 infection

SUMMARY We studied the spontaneous in vitro secretion of anti‐HIV‐1 antibodies by peripheral blood mononuclear cells (PBMC) from HIV‐1‐infected patients. Specific antibody production was detected in supernatants of PBMC cultures using an ELISA; HIV‐1 specificity was confirmed by antigen adsorption a...

Full description

Saved in:
Bibliographic Details
Published in:Clinical and experimental immunology 1991-02, Vol.83 (2), p.197-202
Main Authors: VENDRELL, J. P., SEGONDY, M., DUCOS, J., REYNES, J., HUGUET, M. F., NICOLAS, J. C., SERRE, A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
AIDS/HIV
antibodies
Biological and medical sciences
Cells, Cultured
Gene Products, gag - analysis
HIV Antibodies - analysis
HIV Core Protein p24
HIV Infections - immunology
HIV Seropositivity - immunology
HIV-1 - immunology
HIV‐1
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Leukocytes, Mononuclear - immunology
Medical sciences
Middle Aged
peripheral blood mononuclear cells
secretion
Viral Core Proteins - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:SUMMARY We studied the spontaneous in vitro secretion of anti‐HIV‐1 antibodies by peripheral blood mononuclear cells (PBMC) from HIV‐1‐infected patients. Specific antibody production was detected in supernatants of PBMC cultures using an ELISA; HIV‐1 specificity was confirmed by antigen adsorption and Western blotting. This antibody secretion was found to be an active phenomenon and was not due to a release of plasma antibodies passively adsorbed onto the cell membranes. In all positive supernatants, anti‐HIV‐1‐secreted antibodies were directed against enu‐encoded antigens and many supernatants also contained antibodies to pol‐ and gag‐encoded antigens. PBMC from all HIV‐1‐infected patients tested (140 adults and 18 infants) secreted anti‐HIV‐1 antibodies. This production was found during all the clinical stages of HIV‐1 infection. Our results suggest that this spontaneous HIV‐1‐specific antibody secretion represents a marker of HIV‐1 infection. Detection of these antibodies could be a valuable tool for early confirmation of HIV‐1 infection in neonates born to HIV‐1‐seropositive mothers.
ISSN:0009-9104
1365-2249
DOI:10.1111/j.1365-2249.1991.tb05614.x