Formal analysis of electrogenic sodium, potassium, chloride and bicarbonate transport in mouse colon epithelium

• Published in: British journal of pharmacology 1999-01, Vol.126 (1), p.358-364
• Main Authors: Cuthbert, A W, Hickman, M E, MacVinish, L J
• Format: Article
• Language: English
• Subjects: Acetazolamide - pharmacology; Amiloride - pharmacology; Animals; bicarbonate secretion; Bicarbonates - pharmacokinetics; Biological and medical sciences; Biological Transport - drug effects; Cell physiology; CFTR; chloride secretion; Chlorides - pharmacokinetics; Colforsin - pharmacology; Colon - drug effects; Colon - metabolism; Colon - physiopathology; cystic fibrosis; Cystic Fibrosis - metabolism; Cystic Fibrosis - physiopathology; Diuretics - pharmacology; Electrogenic epithelial transport; Electrophysiology; Epithelium - drug effects; Epithelium - metabolism; Epithelium - physiopathology; forskolin; Fundamental and applied biological sciences. Psychology; Furosemide - pharmacology; In Vitro Techniques; Inorganic Chemicals - pharmacokinetics; loop diuretics; Medical sciences; Membrane and intracellular transports; Membrane Potentials - drug effects; Mice; Mice, Inbred CFTR; Models, Theoretical; Molecular and cellular biology; Pharmacology. Drug treatments; Potassium - pharmacokinetics; potassium secretion; Sodium - pharmacokinetics; sodium absorption; Urinary system
• ISSN: 0007-1188; 1476-5381
• DOI: 10.1038/sj.bjp.0702290

The mammalian colonic epithelium carries out a number of different transporting activities simultaneously, of which more than one is increased following activation with a single agonist. These separate activities can be quantified by solving a set of equations describing these activities, provided some of the dependent variables can be eliminated. Using variations in the experimental conditions, blocking drugs and comparing wild type tissues with those from transgenic animals this has been achieved for electrogenic ion transporting activity of the mouse colon. Basal activity and that following activation with forskolin was measured by short circuit current in isolated mouse colonic epithelia from normal and cystic fibrosis (CF) mice. Using amiloride it is shown that CF colons show increased electrogenic sodium absorption compared to wild type tissues. CF mice had elevated plasma aldosterone, which may be responsible for part or all of the increased sodium absorbtion in CF colons. The derived values for electrogenic chloride secretion and for electrogenic potassium secretion were increased by 13 and 3 fold respectively by forskolin, compared to basal state values for these processes. The loop diuretic, frusemide, completely inhibited electrogenic potassium secretion, but apparently only partially inhibited electrogenic chloride secretion. However, use of bicarbonate‐free solutions and acetazolamide reduced the frusemide‐resistant current, suggesting that electrogenic bicarbonate secretion accounts for the frusemide‐resistant current. It is argued that the use of tissues from transgenic animals is an important adjunct to pharmacological analysis, especially where effects in tissues result in the activation of more than one sort of response. British Journal of Pharmacology (1999) 126, 358–364; doi:10.1038/sj.bjp.0702290