The soybean GH2/4 gene that encodes a glutathione S-transferase has a promoter that is activated by a wide range of chemical agents

• Published in: Plant physiology (Bethesda) 1995-07, Vol.108 (3), p.919-927
• Main Authors: Ulmasov, T. (University of Missouri, Columbia, MO.), Ohmiya, A, Hagen, G, Guilfoyle, T
• Format: Article
• Language: English
• Subjects: 2,4,5-T; 2,4-D; ACTIVADOR ENZIMATICO; ACTIVATEUR D'ENZYME; ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; ANA; Antibodies; ANTICORPS; ANTICUERPOS; ARN MENSAJERO; ARN MESSAGER; AUXINAS; AUXINE; Auxins; Base Sequence; BENCENO; BENZENE; Biological and medical sciences; CHIMIE; Chromatography, Agarose; CITOPLASMA; CODE GENETIQUE; CODIGO GENETICO; CONTENIDO PROTEICO; CYTOPLASME; DNA, Plant; Electrophoresis, Polyacrylamide Gel; ETAPAS DE DESARROLLO; EXPRESION GENICA; EXPRESSION DES GENES; Fundamental and applied biological sciences. Psychology; GENE; Gene Expression Regulation, Plant - drug effects; Gene Regulation and Molecular Genetics; GENES; GLICOSIDASAS; Glutathione Transferase - genetics; GLYCINE MAX; Glycine max - genetics; GLYCOSIDASE; Heavy metals; HERBICIDAS; HERBICIDE; Hypocotyls; Indoleacetic Acids - pharmacology; MEDIO DE CULTIVO; Messenger RNA; METAL LOURD; METALES PESADOS; Metals - pharmacology; MILIEU DE CULTURE; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Nicotiana - genetics; NICOTIANA TABACUM; Oxidative stress; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified; Plants, Toxic; Promoter Regions, Genetic; PURIFICACION; PURIFICATION; QUIMICA; Recombinant Fusion Proteins - genetics; RNA, Messenger - genetics; SECUENCIA NUCLEOTIDICA; Seedlings; SEQUENCE NUCLEOTIDIQUE; Soybeans; STADE DE DEVELOPPEMENT; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; TECHNIQUE IMMUNOLOGIQUE; TECNICAS INMUNOLOGICAS; TENEUR EN PROTEINES; TRANSCRIPCION; TRANSCRIPTION; Transcription. Transcription factor. Splicing. Rna processing; TRANSFERASAS; TRANSFERASE; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Transgenic plants
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.108.3.919

Transcriptional activation of the soybean (Glycine max) GH2/4 gene (also referred to as Gmhsp26-A) and increase in abundance of the GH2/4 mRNA (also referred to as pCE54) have been previously shown to occur following treatment of soybean seedlings with auxins, nonauxin analogs, heavy metals, and a variety of other agents. To determine whether the GH2/4 promoter is responsive to an array of different agents, we have analyzed the inducibility of the GH2/4 promoter of to the beta-glucuronidase reporter gene in transgenic tobacco (Nicotiana tabacum) plants. We have shown that a wide variety of chemical agents induce this promoter in a tissue-specific and concentration-dependent manner. In addition, we have an affinity-purified antibody raised against recombinant GH2/4 protein to show that the GH2/4 protein increases in response to auxin application and is localized in the cytosol of soybean cells. Recombinant GH2/4 protein can be purified to homogeneity on a glutathione-agarose resin, and the purified protein has glutathione S-transferase activity when assayed with the substrate 1-chloro-2,4-dinitrobenzene