Plasma amylin and insulin concentrations in normoglycemic and hyperglycemic cats

The recently discovered pancreatic peptide amylin is postulated to be involved in the pathogenesis of feline diabetes mellitus. However, plasma amylin concentrations in normal and diabetic cats have not yet been published. The aim of the present study was to validate a commercial amylin radioimmunoa...

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Bibliographic Details
Published in:Canadian veterinary journal 1996, Vol.37 (1), p.27-34
Main Authors: Lutz, T.A, Rand, J.S
Format: Article
Language:English
Subjects:
Amyloid - blood
Animals
blood plasma
Cat Diseases - blood
Cats
diabetes mellitus
Diabetes Mellitus - blood
Diabetes Mellitus - veterinary
Female
glucose tolerance
Glucose Tolerance Test - veterinary
hyperglycemia
Hyperglycemia - blood
Hyperglycemia - veterinary
hyperinsulinemia
insulin
Insulin - blood
Islet Amyloid Polypeptide
Male
pancreatic hormones
Radioimmunoassay - veterinary
radioimmunoassays
Reproducibility of Results
secretion
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Summary:The recently discovered pancreatic peptide amylin is postulated to be involved in the pathogenesis of feline diabetes mellitus. However, plasma amylin concentrations in normal and diabetic cats have not yet been published. The aim of the present study was to validate a commercial amylin radioimmunoassay kit for the measurement of feline amylin in unextracted plasma, and to measure plasma amylin concentrations in normal and diabetic cats. The kit had satisfactory specificity, sensitivity, accuracy, and precision, and can be recommended for measurement of feline amylin in unextracted EDTA plasma, when nonspecific binding of plasma samples is used in the calculation of measured amylin concentration. Fasting amylin concentration in cats with normal glucose tolerance was 97 +/- 4 pmol/L. Plasma amylin increased in parallel with insulin after glucose administration in cats with normal and impaired glucose tolerance. In contrast to cats with normal glucose tolerance, cats with impaired glucose tolerance had markedly delayed amylin and insulin secretion. Diabetic cats had basal hypoinsulinemia combined with hyperamylinemia. Hyperamylinemia may lead to reduced insulin secretion and insulin resistance, and contribute to the development of feline diabetes. In conclusion, feline amylin can be measured in unextracted EDTA plasma. Fasting amylin concentrations are approximately 100 pmol/L, and amylin and insulin are cosecreted in cats with normal and impaired glucose tolerance. Increased amylin concentrations may contribute to the development of feline diabetes mellitus.
ISSN:0008-5286