Serodiagnosis of toxoplasma infection using a purified parasite protein (P30)

The major surface protein (P30) of Toxoplasma gondii has been purified by immunoabsorption with anti-P30 monoclonal antibodies linked to a glutardialdehyde activated affinity absorbant. SDS-PAGE analysis of the eluted material followed by silver staining showed only a single band of 30,000 mol wt. W...

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Bibliographic Details
Published in:Clinical and experimental immunology 1985-11, Vol.62 (2), p.262-269
Main Authors: SANTORO, F, AFCHAIN, D, PIERCE, R, CESBRON, J. Y, OVLAQUE, G, CAPRON, A
Format: Article
Language:English
Subjects:
Antigens, Protozoan - isolation & purification
Biological and medical sciences
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Epitopes - analysis
Human protozoal diseases
Humans
Immunoglobulin M - biosynthesis
Infectious diseases
Medical sciences
Membrane Proteins - isolation & purification
Parasitic diseases
Protozoal diseases
Protozoan Proteins
Toxoplasma - immunology
Toxoplasma gondii
Toxoplasmosis
Toxoplasmosis - diagnosis
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Summary:The major surface protein (P30) of Toxoplasma gondii has been purified by immunoabsorption with anti-P30 monoclonal antibodies linked to a glutardialdehyde activated affinity absorbant. SDS-PAGE analysis of the eluted material followed by silver staining showed only a single band of 30,000 mol wt. Western blotting using antibodies from a rabbit immunized with purified P30 against the total Toxoplasma extract separated by SDS-PAGE again revealed an unique antigen of 30,000 daltons. The presence of repeated epitopes within P30 was confirmed by a two-site/one-antibody radiometric assay with the purified protein. Sandwich ELISA procedures with purified P30 clearly demonstrated that all 37 tested patients with acute toxoplasmosis presented significantly high levels of IgM anti-P30 antibodies. In addition, all 40 tested patients with chronic toxoplasma infection also showed high IgG anti-P30 antibody levels. These findings represent an essential step for the development of new reagents for the diagnosis of toxoplasmosis.
ISSN:0009-9104
1365-2249