Little or no repair of cyclobutyl pyrimidine dimers is observed in the organellar genomes of the young Arabidopsis seedling

A Southern-blot-based, site-specific assay for ultraviolet (UV)induced cyclobutyl pyrimidine dimers (CPDs), employing the CPD-specific enzyme T4 endonuclease V, was used to follow the repair of this lesion in particular DNA sequences in 5- to 6-d-old Arabidopsis thaliana seedlings. CPDs, measured as...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1996-05, Vol.111 (1), p.19-25
Main Authors: Chen, J.J. (University of California, Davis, CA.), Jiang, C.Z, Britt, A.B
Format: Article
Language:English
Subjects:
ADN
AGENTES NOCIVOS
Animal, plant and microbial ecology
Applied ecology
ARABIDOPSIS THALIANA
Biological and medical sciences
Cephalopelvic disproportion
Dimers
DNA
DNA damage
DNA probes
DNA repair
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on plants and fungi
FACTEUR NUISIBLE
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genomes
LUMIERE
LUZ
MITOCHONDRIE
MITOCONDRIA
NOYAU CELLULAIRE
NUCLEO
OBSCURIDAD
OBSCURITE
PIRIMIDINAS
PLANTULAS
PLANTULE
PLASTE
PLASTIDIOS
Plastids
PYRIMIDINE
RADIACION ULTRAVIOLETA
RAYONNEMENT ULTRAVIOLET
Ribosomal DNA
SECUENCIA NUCLEOTIDICA
Seedlings
SEQUENCE NUCLEOTIDIQUE
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
Whole Plant, Environmental, and Stress Physiology
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Summary:A Southern-blot-based, site-specific assay for ultraviolet (UV)induced cyclobutyl pyrimidine dimers (CPDs), employing the CPD-specific enzyme T4 endonuclease V, was used to follow the repair of this lesion in particular DNA sequences in 5- to 6-d-old Arabidopsis thaliana seedlings. CPDs, measured as enzyme-sensitive sites, in nuclear sequences were removed rapidly in the light but were repaired slowly, if at all, in the dark. This result was identical to that obtained in prior analyses of CPDs in total cellular DNA. Assay of representative chloroplast and mitochondrial sequences in the same DNA preparations revealed that, in contrast to nuclear sequences, enzyme-sensitive sites are inefficiently eliminated in both the presence and absence of visible light. These observations suggest that Arabidopsis seedlings possess little or no capacity for the repair of CPDs in the organellar genomes. Given the fact that the UV dose employed only marginally affected the growth of the seedlings, we suggest that Arabidopsis seedlings must possess very efficient mechanism(s) for the tolerance of UV-induced DNA damage
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.111.1.19