Early Determinants of H2O2-Induced Endothelial Dysfunction

Reactive oxygen species (ROS) can stimulate nitric oxide (NO•) production from the endothelium by transient activation of endothelial nitric oxide synthase (eNOS). With continued or repeated exposure, NO• production is reduced, however. We investigated the early determinants of this decrease in NO•...

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Bibliographic Details
Published in:Free radical biology & medicine 2006-06, Vol.41 (5), p.810-817
Main Authors: Boulden, Beth M., Widder, Julian D., Allen, Jon C., Smith, Debra A., Al-Baldawi, Ruaa N., Harrison, David G., Dikalov, Sergey I., Jo, Hanjoong, Dudley, Samuel C.
Format: Article
Language:English
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Summary:Reactive oxygen species (ROS) can stimulate nitric oxide (NO•) production from the endothelium by transient activation of endothelial nitric oxide synthase (eNOS). With continued or repeated exposure, NO• production is reduced, however. We investigated the early determinants of this decrease in NO• production. Following an initial H 2 O 2 exposure, endothelial cells responded by increasing NO• production measured electrochemically. NO• concentrations peaked by 10 min with a slow reduction over 30 min. The decrease in NO• at 30 min was associated with a 2.7 fold increase O 2 •− production (p
ISSN:0891-5849
DOI:10.1016/j.freeradbiomed.2006.05.03