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Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato
We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac wa...
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Published in: | The Plant cell 1990-08, Vol.2 (8), p.723-730 |
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container_title | The Plant cell |
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creator | Yoder, John I. |
description | We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac was introduced into a transformant, the number of copies usually remained low in subsequent generations. In one lineage, however, the number of Ac elements increased from one to more than 15 copies in only two generations. DNA gel blot analyses indicated that the amplified elements were not grossly rearranged. Amplified copies of Ac resided at unique sites in the genome, and segregation analysis indicated that these sites were not tightly linked at one genetic locus. Taken together, these observations indicate that the mechanism of Ac amplification is associated with transposition. |
doi_str_mv | 10.1105/tpc.2.8.723 |
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The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac was introduced into a transformant, the number of copies usually remained low in subsequent generations. In one lineage, however, the number of Ac elements increased from one to more than 15 copies in only two generations. DNA gel blot analyses indicated that the amplified elements were not grossly rearranged. Amplified copies of Ac resided at unique sites in the genome, and segregation analysis indicated that these sites were not tightly linked at one genetic locus. Taken together, these observations indicate that the mechanism of Ac amplification is associated with transposition.</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.2.8.723</identifier><identifier>PMID: 1967056</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>Corn ; DNA ; DNA probes ; DNA Transposable Elements ; Gels ; Gene Amplification ; Gene Rearrangement ; Genetic transposition ; Genomes ; Genomics ; Plants ; Plants, Genetically Modified ; Restriction Mapping ; Segregation ; Species Specificity ; Transposons ; Zea mays - genetics</subject><ispartof>The Plant cell, 1990-08, Vol.2 (8), p.723-730</ispartof><rights>Copyright 1990 American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c501t-89085e08cd6674d0bd33ed436b6efb4b05dfadffd25069d13457daa2b90ffa953</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869171$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869171$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,58213,58446</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1967056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoder, John I.</creatorcontrib><title>Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac was introduced into a transformant, the number of copies usually remained low in subsequent generations. In one lineage, however, the number of Ac elements increased from one to more than 15 copies in only two generations. DNA gel blot analyses indicated that the amplified elements were not grossly rearranged. Amplified copies of Ac resided at unique sites in the genome, and segregation analysis indicated that these sites were not tightly linked at one genetic locus. Taken together, these observations indicate that the mechanism of Ac amplification is associated with transposition.</description><subject>Corn</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA Transposable Elements</subject><subject>Gels</subject><subject>Gene Amplification</subject><subject>Gene Rearrangement</subject><subject>Genetic transposition</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Restriction Mapping</subject><subject>Segregation</subject><subject>Species Specificity</subject><subject>Transposons</subject><subject>Zea mays - genetics</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNpVkUtLxTAQhYMovleuhSzEjfQ6SZq0WbgQ8QWKIldwF9Im0Ujb1KRX0F9vteJjNcOcbx7MQWiHwIwQ4IdDX8_orJwVlC2hdcIZzagsH5bHHHLIcsHJGtpI6RkASEHkKlolUhTAxTqa3-neG3wbQ-OdjXrwocPB4eHJ4mvt3y2eR92lPiRdNRafNra13YCP68G_6iFE7LuJeLSdr_E8tGN1C6043SS7_R030f3Z6fzkIru6Ob88Ob7Kag5kyEoJJbdQ1kaIIjdQGcasyZmohHVVXgE3ThvnDOUgpCEs54XRmlYSnNOSs010NM3tF1VrTT1eFnWj-uhbHd9U0F79Vzr_pB7DqyJcSvrZv__dH8PLwqZBtT7Vtml0Z8MiqRJywhhlI3gwgXUMKUXrfnYQUJ8eqNEDRVWpii969-9Zv-z09FHfm_TnNH7w7yjKoFCsFHK0iX0AgMSQcw</recordid><startdate>19900801</startdate><enddate>19900801</enddate><creator>Yoder, John I.</creator><general>American Society of Plant Physiologists</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19900801</creationdate><title>Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato</title><author>Yoder, John I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c501t-89085e08cd6674d0bd33ed436b6efb4b05dfadffd25069d13457daa2b90ffa953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Corn</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA Transposable Elements</topic><topic>Gels</topic><topic>Gene Amplification</topic><topic>Gene Rearrangement</topic><topic>Genetic transposition</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Restriction Mapping</topic><topic>Segregation</topic><topic>Species Specificity</topic><topic>Transposons</topic><topic>Zea mays - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoder, John I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoder, John I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1990-08-01</date><risdate>1990</risdate><volume>2</volume><issue>8</issue><spage>723</spage><epage>730</epage><pages>723-730</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>We have found that the maize transposable element Activator (Ac) can rapidly proliferate when transformed into tomato plants. The fate of transposed Ac elements in self-pollinated progeny of independent transgenic tomato plants was examined by DNA gel blot hybridizations. When a single copy of Ac was introduced into a transformant, the number of copies usually remained low in subsequent generations. In one lineage, however, the number of Ac elements increased from one to more than 15 copies in only two generations. DNA gel blot analyses indicated that the amplified elements were not grossly rearranged. Amplified copies of Ac resided at unique sites in the genome, and segregation analysis indicated that these sites were not tightly linked at one genetic locus. Taken together, these observations indicate that the mechanism of Ac amplification is associated with transposition.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>1967056</pmid><doi>10.1105/tpc.2.8.723</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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source | Alma/SFX Local Collection; JSTOR |
subjects | Corn DNA DNA probes DNA Transposable Elements Gels Gene Amplification Gene Rearrangement Genetic transposition Genomes Genomics Plants Plants, Genetically Modified Restriction Mapping Segregation Species Specificity Transposons Zea mays - genetics |
title | Rapid Proliferation of the Maize Transposable Element Activator in Transgenic Tomato |
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