Calmodulin isoforms differentially enhance the binding of cauliflower nuclear proteins and recombinant TGA3 to a region derived from the Arabidopsis Cam-3 promoter

Many stimuli increase cytoplasmic Ca2+ concentrations as an early signal transduction event and alter the patterns of nuclear gene transcription, but the mechanisms by which Ca2+ signals are transduced to the nucleus are not known. This article shows that at least four DNA binding proteins from caul...

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Bibliographic Details
Published in:The Plant cell 1996-06, Vol.8 (6), p.1069-1077
Main Authors: Szymanski, D.B. (University of Illinois, Urbana, IL.), Liao, B, Zielinski, R.E
Format: Article
Language:English
Subjects:
ADN
Arabidopsis
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins
ARABIDOPSIS THALIANA
Base Sequence
Basic-Leucine Zipper Transcription Factors
Brassica - metabolism
Brassica oleracea
Calmodulin - biosynthesis
Calmodulin - metabolism
Calmodulin-Binding Proteins - metabolism
CALMODULINA
CALMODULINE
Carrier proteins
Cauliflower
Cell Nucleus - metabolism
DNA
DNA Footprinting
DNA probes
DNA, Plant - chemistry
DNA, Plant - metabolism
DNA-Binding Proteins - metabolism
Gels
Molecular Sequence Data
Nuclear proteins
Nuclear Proteins - metabolism
Phosphoprotein Phosphatases - metabolism
Plant cells
Plant Proteins - metabolism
POLIMORFISMO BIOQUIMICO
POLYMORPHISME BIOCHIMIQUE
Promoter regions
Promoter Regions, Genetic
Protein isoforms
PROTEINAS AGLUTINANTES
PROTEINE DE LIAISON
Recombinant Proteins - biosynthesis
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Transcription factors
Transcription Factors - metabolism
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Summary:Many stimuli increase cytoplasmic Ca2+ concentrations as an early signal transduction event and alter the patterns of nuclear gene transcription, but the mechanisms by which Ca2+ signals are transduced to the nucleus are not known. This article shows that at least four DNA binding proteins from cauliflower nuclear extracts are also calmodulin (CaM) binding proteins. CaM enhances the binding of these proteins to a C/G-box sequence element in the Arabidopsis Cam-3 promoter. Binding to the C/G-box is enhanced preferentially by the CaM isoform encoded by Cam-3. However, it is not clear whether the effect is mediated directly by CaM or indirectly through the activity of a CaM-regulated protein phosphatase. CaM also binds recombinant TGA3 and enhances its binding to the same Cam-3 promoter element. These results are consistent with the idea that a Ca2+-mediated signaling pathway eliciting some changes in gene expression may consist of CaM, or a structurally related Ca2+ binding protein, and transcription factors
ISSN:1040-4651
1532-298X
DOI:10.1105/tpc.8.6.1069