Mechanisms underlying cannabinoid inhibition of presynaptic Ca2+ influx at parallel fibre synapses of the rat cerebellum

• Published in: The Journal of physiology 2004-05, Vol.557 (1), p.159-174
• Main Authors: Daniel, H., Rancillac, A., Crepel, F.
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - metabolism; Aniline Compounds; Animals; Benzoxazines; Calcium - metabolism; Calcium Channel Blockers - pharmacology; Calcium Channels - drug effects; Calcium Channels - metabolism; Cannabinoids - pharmacology; Cerebellum - cytology; Cerebellum - drug effects; Cerebellum - metabolism; Cognitive Sciences; Depression, Chemical; Electrophysiology; Fluorometry; GTP-Binding Protein alpha Subunits, Gi-Go - metabolism; In Vitro Techniques; Ion Channel Gating - drug effects; Life Sciences; Male; Membrane Potentials - physiology; Mitogen-Activated Protein Kinases - metabolism; Morpholines - pharmacology; Naphthalenes - pharmacology; Nerve Fibers - drug effects; Nerve Fibers - metabolism; Neurons and Cognition; Patch-Clamp Techniques; Pertussis Toxin - pharmacology; Rats; Rats, Sprague-Dawley; Receptor, Cannabinoid, CB1 - drug effects; Receptor, Cannabinoid, CB1 - metabolism; Receptors, Presynaptic - drug effects; Receptors, Presynaptic - metabolism; Research Papers; Xanthenes
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.2004.063263

Activation of CB1 cannabinoid receptors in the cerebellum acutely depresses excitatory synaptic transmission at parallel fibre–Purkinje cell synapses by decreasing the probability of glutamate release. This depression involves the activation of presynaptic 4-aminopyridine-sensitive K + channels by CB1 receptors, which in turn inhibits presynaptic Ca 2+ influx controlling glutamate release at these synapses. Using rat cerebellar frontal slices and fluorometric measures of presynaptic Ca 2+ influx evoked by stimulation of parallel fibres with the fluorescent dye fluo-4FF, we tested whether the CB1 receptor-mediated inhibition of this influx also involves a direct inhibition of presynaptic voltage-gated calcium channels. Since various physiological effects of CB1 receptors appear to be mediated through the activation of PTX-sensitive proteins, including inhibition of adenylate cyclases, activation of mitogen-activated protein kinases (MAPK) and activation of G protein-gated inwardly rectifying K + channels, we also studied the potential involvement of these intracellular signal transduction pathways in the cannabinoid-mediated depression of presynaptic Ca 2+ influx. The present study demonstrates that the molecular mechanisms underlying the CB1 inhibitory effect involve the activation of the PTX-sensitive G i /G o subclass of G proteins, independently of any direct effect on presynaptic Ca 2+ channels (N, P/Q and R (SNX-482-sensitive) types) or on adenylate cyclase or MAPK activity, but do require the activation of G protein-gated inwardly rectifying (Ba 2+ - and tertiapin Q-sensitive) K + channels, in addition to 4-aminopyridine-sensitive K + channels.